MS Quiz

MS Quiz for everyone:

A.) Can you tell me the charge, mass and name of this human protein from a top down experiment?

All information’s you need to calculate the protein mass are given in Figure 1.

Figure 1, Orbitrap XL, FT, resolution: 100.000

The following tool can be helpfull: http://web.expasy.org/tagident/

(Mw range in TagIdent should be set to 0.0001%)

Orbitrap XL, FT, resolution: 100.000 Zoom Image
Orbitrap XL, FT, resolution: 100.000

B.) Can you tell me the peptide sequence of this MS2 experiment? To which human protein does it belong to?

All information’s you need to calculate the peptide sequence are given in Figure 2 and Table 1 (M+H+: m/z: 421Da, charge: +2).

You can use following program for the search: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins

Figure 2 

<p>MS/MS LTQ</p> Zoom Image

MS/MS LTQ

Table1: single charged ions:

y-ions

 

b-ions

 

y(1)

175.1190

n/a

 

y(2)

232.1404

b(2)

263.0882

y(3)

319.1724

b(3)

394.1278

y(4)

447.2310

n/a

 

y(5)

578.2715

n/a

 

y(6)

709.3120

n/a

 

 
 
 
 
 
 
  
 

If you are applying for a position in my MS lab, the right answers are mandatory:)

Good luck!

 
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