List  of Publications

 

2023

Ravid Lustig L, Sampath Kumar A, Schwämmle T, Dunkel I, Noviello G, Limberg E, Weigert R, Pacini G, Buschow R, Ghauri A, Stötzel M, Wittler L, Meissner A, Schulz EG (2023) GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers. Nat Cell Biol. 2023 Nov;25(11):1704-1715. doi: 10.1038/s41556-023-01266-x. Epub 2023 Nov 6. PMID: 37932452.

Modhiran N, Lauer SM, Amarilla AA, Hewins P, Lopes van den Broek SI, Low YS, Thakur N, Liang B, Nieto GV, Jung J, Paramitha D, Isaacs A, Sng JDJ, Song D, Jørgensen JT, Cheuquemilla Y, Bürger J, Andersen IV, Himelreichs J, Jara R, MacLoughlin R, Miranda-Chacon Z, Chana-Cuevas P, Kramer V, Spahn C, Mielke T, Khromykh AA, Munro T, Jones ML, Young PR, Chappell K, Bailey D, Kjaer A, Herth MM, Jurado KA, Schwefel D, Rojas-Fernandez A, Watterson D. (2023) A nanobody recognizes a unique conserved epitope and potently neutralizes SARS-CoV-2 omicron variants. iScience. 2023 Jun 9;26(7):107085. doi: 10.1016/j.isci.2023.107085. eCollection 2023 Jul 21. PMID: 37361875.

Smirnova J, Loerke J, Kleinau G, Schmidt A, Bürger J, Meyer EH, Mielke T, Scheerer P, Bock R, Spahn CMT, Zoschke R (2023) Structure of the actively translating plant 80S ribosome at 2.2 Å resolution. Nat Plants. 2023 May 8. doi: 10.1038/s41477-023-01407-y. Online ahead of print. PMID: 37156858.

Qin B, Lauer SM, Balke A, Vieira-Vieira CH, Bürger J, Mielke T, Selbach M, Scheerer P, Spahn CMT, Nikolay R (2023) Cryo-EM captures early ribosome assembly in action. Nat Commun. 2023 Feb 17;14(1):898. doi: 10.1038/s41467-023-36607-9. PMID: 36797249.

Mensah MA, Niskanen H, Magalhaes AP, Basu S, Kircher M, Sczakiel HL, Reiter AMV, Elsner J, Meinecke P, Biskup S, Chung BHY, Dombrowsky G, Eckmann-Scholz C, Hitz MP, Hoischen A, Holterhus PM, Hülsemann W, Kahrizi K, Kalscheuer VM, Kan A, Krumbiegel M, Kurth I, Leubner J, Longardt AC, Moritz JD, Najmabadi H, Skipalova K, Snijders Blok L, Tzschach A, Wiedersberg E, Zenker M, Garcia-Cabau C, Buschow R, Salvatella X, Kraushar ML, Mundlos S, Caliebe A, Spielmann M, Horn D, Hnisz D (2023) Aberrant phase separation and nucleolar dysfunction in rare genetic diseases. Nature. 2023 Feb;614(7948):564-571. doi: 10.1038/s41586-022-05682-1. Epub 2023 Feb 8. PMID: 36755093.

Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM, Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL, Meissner A. (2023) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation. Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077. PMID: 36719724.

Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM, Binting L, Lauer S, Graziadei A, Klingen R, Gotthold C, Bürger J, Bracht T, Sitek B, Jan Lebbink R, Malyshkina A, Mielke T, Rappsilber J, Spahn CM, Voigt S, Trilling M, Schwefel D (2023) Structural mechanism of CRL4-instructed STAT2 degradation via a novel cytomegaloviral DCAF receptor. EMBO J. 2023 Mar 1;42(5):e112351. doi: 10.15252/embj.2022112351. Epub 2023 Feb 10. PMID: 36762436.

 

2022

Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, Münster-Wandowski A, Fauler B, Mielke T, Selbach M, Landthaler M, Spahn CMT, Tarabykin V, Ohler U, Kraushar ML (2022) A critical period of translational control during brain development at codon resolution. Nat Struct Mol Biol. 2022 Dec;29(12):1277-1290. doi: 10.1038/s41594-022-00882-9. PMID: 36482253.

López-Anguita N, Gassaloglu SI, Stötzel M, Bolondi A, Conkar D, Typou M, Buschow R, Veenvliet VL and Bulut-Karslioglu, A (2022) Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids. Development, Oct 15, 149(20):dev200679. doi: 10.1242/dev.200679.

Hilal T, Killam BY, Grozdanović M, Dobosz-Bartoszek M, Loerke J, Bürger J, Mielke T, Copeland PR, Simonović M and Spahn CMT (2022) Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon. Science, Jun 17;376(6599):1338-1343. doi: 10.1126/science.abg3875. PMID: 35709277.

Rosebrock D, Arora S, Mutukula N, Volkman R, Gralinska E, Balaskas A, Hernández AA, Buschow R, Brändl B, Mueller FJ, Arndt PJ, Vingron M, Elkabetz Y (2022) Enhanced cortical neural stem cell identity through short SMAD/WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol. 2022 Jun;24(6):981-995. doi: 10.1038/s41556-022-00929-5. PMID: 35697781.

Asimi V, Kumar AS, Niskanen H, Riemenschneider C, Hetzel S, Naderi J, Fasching N, Popitsch N, Du M, Kretzmer M, Smith ZD, Weigert R, Walther M, Mamde S, Meierhofer D, Wittler L, Buschow R, Timmermann B, Cisse II, Ameres SL, Meissner A and Hnisz D (2022) Hijacking of transcriptional condensates by endogenous retroviruses. Nat Genet. 2022 Jul 21. doi: 10.1038/s41588-022-01132-w. PMID: 35864192.

Kornak U, Saha N, Keren B, Neumann A, Taylor Tavares AL, Piard J, Kopp J, Rodrigues Alves JG, Rodríguez de los Santos M, El Choubassi N, Ehmke N, Jäger M, Spielmann M, Pantel JT, Lejeune E, Fauler B, Mielke T, Hecht J, Meierhofer D, Strom TM, Laugel V, Brice A, Mundlos S, Bertoli-Avella A, Bauer P, Heyd F, Boute O, Dupont J, Depienne C, Van Maldergem L and Fischer-Zirnsak B (2022) BDU13 Progeria: Alternative splicing of BUD13 determines the severity of a developmental disorder with lipodystrophy and progeroid features. Genet Med. 2022 Jun 6:S1098-3600(22)00759-6. doi: 10.1016/j.gim.2022.05.004. PMID: 35670808.

Hochmair J, Exner C, Franck M, Dominguez-Baquero1 A, Diez L, Brognaro H, Kraushar ML, Mielke T, Radbruch H, Kaniyappan S, Falke S, Mandelkow E, Betzel C, Wegmann S (2022) Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates. EMBO J. 2022 Jun 1;41(11):e108882. doi: 10.15252/embj.2021108882. PMID: 35298090.

 

2021

Heyder NA, Kleinau G, Speck D, Schmidt A, Paisdzior S, Szczepek M, Bauer B, Koch A, Gallandi M, Kwiatkowski D, Bürger J, Mielke T, Beck-Sickinger AG, Hildebrand PW, Spahn CMT, Hilger D, Schacherl M, Biebermann H, Hilal T, Kühnen P, Kobilka BK, Scheerer P (2021) Structures of active melanocortin-4 receptor−Gs-protein complexes with NDP-α-MSH and setmelanotide. Cell Res. 2021 Sep 24. doi: 10.1038/s41422-021-00569-8. Online ahead of print. PMID: 34561620.

Banchenko S, Krupp F, Gotthold C, Bürger J, Graziadei A, O'Reilly FJ, Sinn L, Ruda O, Rappsilber J, Spahn CMT, Mielke T, Taylor IA, Schwefel D (2021) Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses. PLoS Pathog 2021 Aug 2;17(8):e1009775. doi: 10.1371/journal.ppat.1009775. eCollection 2021 Aug. PMID: 34561620.

Bothe M, Buschow R, Meijsing SH (2021) Glucocorticoid signaling induces transcriptional memory and universally reversible chromatin changes. Life Sci Alliance. 2021 Aug 26;4(10):e202101080. doi: 10.26508/lsa.202101080. PMID: 34446533.

Labarde, A, Jakutyte, L, Billaudeau, C, Fauler, B, López-Sanz, M, Ponien, P, Jacquet, E, Mielke, T, Ayora, S, Carballido-López, R and Tavares,  P (2021) Temporal compartmentalization of viral infection in bacterial cells. Proc Natl Acad Sci U S A. 2021 Jul 13;118(28):e2018297118. doi: 10.1073/pnas.2018297118. PMID: 34244425.

Jaworski, P, Zyla-Uklejewicz, D, Nowaczyk-Cieszewska, M, Donczew, R, Mielke, T, Weigel, C, Zawilak-Pawlik, A (2021) Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly. Int J Mol Sci. 2021 Jun 21;22(12):6643. doi: 10.3390/ijms22126643. PMID: 34205762.

Pivovarova-Ramich, O, Loske, J, Hornemann, S., Markova, M, Seebeck, N, Rosenthal, A, Klauschen, F, Castro, JP, Buschow, R, Grune, T, Lange, V, Rudovich, N and Ouwens, DM (2021) Hepatic Wnt1 Inducible Signaling Pathway Protein 1 (WISP-1/CCN4) Associates with Markers of Liver Fibrosis in Severe Obesity. Cells 2021 Apr 29;10(5):1048. doi: 10.3390/cells10051048. PMID: 33946738.

Haggerty, C, Kretzmer, H, Riemenschneider, C, Kumar, AS, Mattei, AL, Bailly, N, Gottfreund, J, Giesselmann, P, Weigert, R, Brändl, B, Giehr, P, Buschow, R, Galonska, C, von Meyenn, F, Pappalardi, MB, McCabe, MT, Wittler, L, Giesecke-Thiel, C, Mielke, T, Meierhofer, D, Timmermann, B, Müller, FJ, Walter, J, Meissner A. Dnmt1 has de novo activity targeted to transposable elements. Nat Struct Mol Biol. 2021 Jul;28(7):594-603. doi: 10.1038/s41594-021-00603-8. Epub 2021 Jun 17. PMID: 34140676.

Pooryasin, A, Maglione, M, Schubert, M, Matkovic-Rachid, T, Hasheminasab, SM, Pech, U, Fiala, A, Mielke, T and Sigrist SJ (2021) Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila. Nat Commun. 2021 Mar 26;12(1):1932. doi: 10.1038/s41467-021-22180-6. PMID: 33771998.

Bolondi A, Haut L, Gassaloglu SI, Burton P, Kretzmer H, Buschow R, Meissner A, Herrmann BG, Veenvliet JV. (2021) Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis. Bio Protoc. 2021 Jun 5;11(11):e4042. doi: 10.21769/BioProtoc.4042. PMID: 34250208.

Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira, CH, Mielke T, Bürger J, Loerke J, Amikura K, Flügel, T, Ueda T, Selbach, M, Deuerling E and Spahn CMT (2021) Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. Mol. Cell, 2021 Mar 18;81(6):1200-1215.e9. doi: 10.1016/j.molcel.2021.02.006. PMID: 33639093.

Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Günnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Münster-Wandowski A, Bürger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M and Spahn CMT (2021) Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit. Mol Cell 2021 Jan 21;81(2):304-322.e16. doi: 10.1016/j.molcel.2020.11.037. PMID: 33357414.

Said N, Hilal T, Sunday ND, Khatri A, Bürger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I and Wahl MC (2021) Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ. Science 2021 Jan 1;371(6524):eabd1673. doi: 10.1126/science.abd1673. PMID: 33243850.

 

2020

Veenvliet JV, Bolondi A, Kretzmer H, Haut L, Scholze-Wittler M, Schifferl D, Koch F, Guignard L, Kumar AS, Pustet M, Heimann S, Buschow R, Wittler L, Timmermann B, Meissner A, Herrmann BG. (2020) Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. Science. 2020 Dec 11;370(6522):eaba4937. doi: 10.1126/science.aba4937. PMID: 33303587.

Xiao Y, Rabien A, Buschow R, Amstislavskiy V, Busch J, Kilic E, Villegas S, Timmermann B, Schütte M, Mielke T, Yaspo M-L, Jung K and Meierhofer D (2020) Endocytosis-Mediated Replenishment of Amino Acids Favors Cancer Cell Proliferation and Survival in Chromophobe Renal Cell Carcinoma. Cancer Res. 2020 Dec 15;80(24):5491-5501. doi: 10.1158/0008-5472.CAN-20-1998. Epub 2020 Oct 28. PMID: 33115803.

Ataç B, Kiss FM, Lam T, Fauler B, Edler C, Hu P, Tao TP, Jädicke M, Rütschle I, Azar RP, Youngquist S, Mielke T, Marx U, Lauster R, Lindner G, DiColandrea T. (2020) The microfollicle: a model of the human hair follicle for in vitro studies. In Vitro Cell Dev Biol Anim. 2020 Dec;56(10):847-858. doi: 10.1007/s11626-020-00513-x. PMID: 33170472.

Huang YH, Hilal T, Loll B, Bürger J, Mielke T, Böttcher C, Said N, Wahl MC (2020) Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis. Mol Cell. Sep 17;79(6):1024-1036.e5. doi: 10.1016/j.molcel.2020.08.010 Epub 2020 Aug 31. PMID: 32871103.

Bischoff P, Kornhuber M, Dunst S, Zell J, Fauler B, Mielke T, Taubenberger AV, Vogl S, Guck J, Oelgeschläger M and Schönfelder G (2020) Estrogens Determine Adherens Junction Organization and E-Cadherin Clustering in Breast Cancer Cells via Amphiregulin. iScience. 2020 Oct 15;23(11):101683. doi: 10.1016/j.isci.2020.101683. eCollection 2020 Nov 20. PMID: 33163938.

Kamel M, Pavulraj S, Fauler B, Mielke T and Azab W (2020) Herpesviruses exploit the extracellular matrix of mononuclear cells to ensure transport to target cells. iScience 2020 Oct 23;23(10):101615. doi: 10.1016/j.isci.2020.101615. Epub 2020 Sep 28. PMID: 33015592.

Radon C, Mittelstädt G, Duffus BR, Bürger J, Hartmann T, Mielke T, Teutloff C, Leimkühler D and Wendler P (2020). Cryo-EM Structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus Formate Dehydrogenase. Nat Commun. 2020 Apr 20;11(1):1912., doi: 10.1038/s41467-020-15614-0. PMID: 32313256.

Kölsch A, Radon C, Golub M, Baumert A, Bürger J, Mielke T, Lisdat F, Feoktystov A, Pieper J, Zouni A, Wendler P. (2020) Current limits of structural biology: The transient interaction between cytochrome c 6 and photosystem I. Curr Res Struct Biol. 2020 Aug 26;2:171-179. doi: 10.1016/j.crstbi.2020.08.003. eCollection 2020. PMID: 34235477.

Lunelli M, Kamprad A, Bürger J, Mielke T, Spahn CMT and Kolbe M (2020). Cryo-EM structure of the Shigella type 3 needle complex. PLoS Pathog. 2020 Feb 24;16(2):e1008263. doi: 10.1371/journal.ppat.1008263. eCollection. PMID: 32092125.

Nowaczyk-Cieszewska M, Zyla-Uklejewicz D, Noszka M, Jaworski P, Mielke T, Zawilak-Pawlik A (2020). The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication. Mol Microbiol. 2020 Feb;113(2):338-355. doi: 10.1111/mmi.14423. PMID: 31715026.

 

2019

Ignatiou A, Brasilès S, El Sadek Fadel M, Bürger J, Mielke T, Topf M, Tavares P, Orlova EV(2019). Structural transitions during the scaffolding-driven assembly of a viral capsid. Nat Commun. 2019 Oct 24;10(1):4840. doi: 10.1038/s41467-019-12790-6. PMID: 31649265.

Brandt R, Sell T, Lüthen M, Uhlitz F, Klinger B, Riemer P, Giesecke-Thiel C, Schulze S, El-Shimy IA, Kunkel D, Fauler B, Mielke T, Mages N, Herrmann BG, Sers C, Blüthgen N, Morkel M. (2019). Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium. Nat Commun. 2019 Jul 2;10(1):2919. doi: 10.1038/s41467-019-10954-y. PMID: 31266962.

Krupp F, Said N, Huang YH, Loll B, Bürger J, Mielke T, Spahn CMT and Wahl MC (2019) Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor. Mol Cell. 74, 142-157.e5. doi: 10.1016/j.molcel.2019.01.016. PMID: 30795892.

 

2018

Flis J, Holm M, Rundlet EJ, Loerke J, Hilal T, Dabrowski M, Bürger J, Mielke T, Blanchard SC, Spahn CMT and Budkevich TV (2018) tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis. Cell Rep. 2018 Dec 4;25(10):2676-2688.e7. doi: 10.1016/j.celrep.2018.11.040. PMID: 30517857.

Qureshi BM, Behrmann E, Schöneberg J, Loerke J, Bürger J, Mielke T, Giesebrecht J, Noé F, Hofmann KP, Spahn CMT and Heck M (2018) It takes two transducins to activate the cGMP-phosphodiesterase 6 in retinal rods. Open Biol. 2018 Aug;8(8). pii: 180075. doi: 10.1098/rsob.180075. PMID: 30068566.

Börner C, Scheerer C, Buschow R, Chiantera V, Sehouli J, Mechsner S. (2018) Pain Mechanisms in Peritoneal Diseases Might Be Partially Regulated by Estrogen. Reprod Sci. 2018 Mar;25(3):424-434. doi: 10.1177/1933719117715126. Epub 2017 Jun 29. PMID: 28659008.

Jaworski P, Donczew R, Mielke T, Weigel C, Stingl K, Pawlik AM (2018) Structure and function of the Campylobacter jejuni chromosome replication origin. Frontiers in Microbiology 9, Article 1533. doi: 10.3389/fmicb.2018.01533.

Nikolay R, Hilal T, Qin B, Mielke T, Bürger J, Loerke J, Textoris-Taube K, Nierhaus KH, Spahn CMT (2018) Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution. Molecular Cell 70, 881-893. Doi:10.1016/j.molcel.2018.05.003. Epub 2018 Jun 7. PMID: 29883607.

Devulapally PR, Bürger J, Mielke T, Konthur Z, Lehrach H, Yaspo ML, Glökler J, Warnatz HJ (2018) Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes. Genome Med. 2018 Apr 27;10(1):34. doi: 10.1186/s13073-018-0542-5. PMID: 29703216.

Qureshi BM, Schmidt A, Behrmann E, Bürger J, Mielke T, Spahn CMT, Heck M, Scheerer P (2018) Mechanistic insights into the role of prenyl-binding protein PrBP/δ in membrane dissociation of phosphodiesterase 6. Nat Commun. Jan 8;9(1):90. doi: 10.1038/s41467-017-02569-y. PMID: 29311697.

 

2017

Ehmke N, Graul-Neumann L, Smorag L, Koenig R, Segebrecht L, Magoulas P, Scaglia F, Kilic E, Hennig AF, Adolphs N, Saha N, Fauler B, Kalscheuer VM, Hennig F, Altmüller J, Netzer C, Thiele H, Nürnberg P, Yigit G, Jäger M, Hecht J, Krüger U, Mielke T, Krawitz PM, Horn D, Schuelke M, Mundlos S, Bacino CA, Bonnen PE, Wollnik B, Fischer-Zirnsak B, Kornak U (2017) De Novo Mutations in SLC25A24 Cause a Craniosynostosis Syndrome with Hypertrichosis, Progeroid Appearance, and Mitochondrial Dysfunction. Am J Hum Genet. 2017 Nov 2;101(5):833-843. doi: 10.1016/j.ajhg.2017.09.016.

Reddy-Alla S, Böhme MA, Reynolds E, Beis C, Grasskamp AT, Mampell MM, Maglione M, Jusyte M, Rey U, Babikir H, McCarthy AW, Quentin C, Matkovic T, Bergeron DD, Mushtaq Z, Göttfert F, Owald D, Mielke T, Hell SW, Sigrist SJ, Walter AM (2017) Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission. Neuron 95, 1350-1364.e12. doi: 10.1016/j.neuron.2017.08.016. Epub 2017 Aug 31.

Matz P, Wruck W, Fauler B, Herebian D, Mielke T, Adjaye J (2017) Footprint-free human fetal foreskin derived iPSCs: A tool for modeling hepatogenesis associated gene regulatory networks. Sci Rep. 2017 Jul 24;7(1):6294. doi: 10.1038/s41598-017-06546-9.

Said N, Krupp F, Anedchenko E, Santos K, Dybkov O, Huang Y-H, Lee C-T, Loll B, Behrmann E, Bürger J, Mielke T, Loerke J, Urlaub H, Spahn CM, Weber G, Wahl, M (2017) Structural basis for λN-dependent processive transcription antitermination. Nat Microbiol. 2017 Apr 28;2:17062. doi: 10.1038/nmicrobiol.2017.62.

Lorenz C, Lesimple P, Bukowiecki R, Zink A, Inak G, Mlody B, Singh M, Semtner M, Mah N, Auré K, Leong M, Zabiegalov O, Lyras EM, Pfiffer V, Fauler B, Eichhorst J, Wiesner B, Huebner N, Priller J, Mielke T, Meierhofer D, Izsvák Z, Meier JC, Bouillaud F, Adjaye J, Schuelke M, Wanker EE, Lombès A, Prigione A (2017) Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders. Cell Stem Cell. 2017 Jan 25. pii: S1934-5909(16)30469-6. doi: 10.1016/j.stem.2016.12.013. [Epub ahead of print].

 

2016

Hilal T, Yamamoto H, Loerke J, Bürger J, Mielke T, Spahn CM (2016) Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution. Nat Commun. 2016 Dec 20;7:13521. doi: 10.1038/ncomms13521.

Arumughan A, Roske Y, Barth C, Forero LL, Bravo-Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez-Garcia E, Rocks O, Panáková D, Heinemann U, Wanker EE (2016) Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. Nat. Commun. 7:13047.

Jaworski P, Donczew R, Mielke T, Thiel M, Oldziej S, Weigel C, Zawilak-Pawlik A (2016) Unique and Universal Features of Epsilonproteobacterial Origins of Chromosome Replication and DnaA-DnaA Box Interactions. Front Microbiol. 7:1555

Gupta VK, Pech U, Bhukel A, Fulterer A, Ender A, Mauermann SF, Andlauer TF, Antwi-Adjei E, Beuschel C, Thriene K, Maglione M, Quentin C, Bushow R, Schwärzel M, Mielke T, Madeo F, Dengjel J, Fiala A, Sigrist SJ (2016) Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release. PLoS Biol. 14(9):e1002563. doi: 10.1371/journal.pbio.1002563

Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke L, Ismer J, Hildebrand PW, Scheerer P, Bürger J, Mielke T, Spahn CMT (2016) Structures of ribosome-bound initiation factor 2 reveal mechanism of subunit association. Sci. Adv. 2(3):e1501502. doi: 10.1126/sciadv.1501502

 

2015

Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Bürger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT (2015) Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA. EMBO J. 34, 3042-58.

Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Bürger J, Mielke T, Scheerer P, Spahn CMT (2015) Structural snapshots of actively translating human ribosomes. Cell 161, 845-57.

Chaban Y, Lurz R, Brasilès S, Cornilleau C, Karreman M, Zinn-Justin S, Tavares P, Orlova EV (2015) Structural rearrangements in the phage head-to-tail interface during assembly and infection. Proc Natl Acad Sci U S A 112, 7009-14.

van der Sluis EO, Bauerschmitt H, Becker T, Mielke T, Frauenfeld J, Berninghausen O, Neupert W, Herrmann JM, Beckmann R (2015) Parallel Structural Evolution of Mitochondrial Ribosomes and OXPHOS Complexes. Genome Biol Evol 7, 1235-51.

Bielmann R, Habann M, Eugster MR, Lurz R, Calendar R, Klumpp J, Loessner MJ (2015) Receptor binding proteins of Listeria monocytogenes bacteriophages A118 and P35 recognize serovar-specific teichoic acids. Virology 477, 110-8.

Hammerl JA, Roschanski N, Lurz R, Johne R, Lanka E, Hertwig S (2015) The Molecular Switch of Telomere Phages: High Binding Specificity of the PY54 Cro Lytic Repressor to a Single Operator Site. Viruses 7, 2771-93.

Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM (2015) Cryo-EM of ribosomal 80S complexes with termination factors reveals the translocated Cricket Paralysis Virus IRES. Mol Cell. 57, 422-32.

 

2014

Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Bürger J, Mielke T and Spahn CMT (2014) Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA. Nat Struct Mol Biol. 21, 721-7.

Röthlein C, Miettinen MS, Borwankar T, Bürger J, Mielke T, Kumke MU and Ignatova Z (2014) Architecture of polyglutamine-containing fibrils from time-resolved fluorescence decay. J Biol Chem. 289, 26817-28.

Budkevich TV, Giesebrecht J, Behrmann E, Loerke J, Ramrath DJF, Mielke T, Ismer J, Hildebrand PW, Tung CS, Nierhaus KH, Sanbonmatsu KY and Spahn CMT (2014) Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement. Cell 158, 121-31.

Donczew R, Mielke T, Jaworski P, Zakrzewska-Czerwińska J and Zawilak-Pawlik A (2014) Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions. J Mol Biol 426, 2769-82.

Barucker C, Harmeier A, Weiske J, Fauler B, Albring KF, Prokop S, Hildebrand P, Lurz R, Heppner FL, Huber O, Multhaup G. (2014) Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem 289, 20182-91.

Shah T, Hegde BG, Morén B, Behrmann E, Mielke T, Moenke G, Spahn CMT, Lundmark R, Daumke O and Langen R (2014) Structural insights into membrane interaction and caveolar targeting of dynamin-like EHD2. Structure 22, 409-20.

Auzat I, Petitpas I, Lurz R, Weise F, Tavares P (2014) A touch of glue to complete bacteriophage assembly: the tail-to-head joining protein (THJP) family. Mol Microbiol 91, 1164-78.

Evrin C, Fernández-Cid A, Riera A, Zech J, Clarke P, Herrera MC, Tognetti S, Lurz R, Speck C (2014) The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res 42, 2257-69.

Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, Renouard M, Huet A, Decottignies P, Davidson AR, Breyton C, Boulanger P (2014) Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components. J Virol 88, 1162-74.

Flayhan A, Vellieux FM, Lurz R, Maury O, Contreras-Martel C, Girard E, Boulanger P, Breyton C (2014) Crystal structure of pb9, the distal tail protein of bacteriophage T5: a conserved structural motif among all siphophages. J Virol 88, 820-8.

 

2013

Ramrath DJF, Lancaster L, Sprink T, Mielke T, Loerke J, Noller HF and Spahn CMT (2013) Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation. Proc Natl Acad Sci USA 110, 20964-20969.

Klatt S, Hartl D, Fauler B, Gagoski D, Castro-Obrego S and Konthur Z (2013) Generation and Characterization of a Leishmania tarentolae Strain for Site-Directed in Vivo Biotinylation of Recombinant Proteins. J Proteome Res 12, 5512-5519.

Matkovic T, Siebert M, Knoche E, Depner H, Mertel S, Owald D, Schmidt M, Thomas U, Sickmann A, Kamin D, Hell SW, Bürger J, Hollmann C, Mielke T, Wichmann C and Sigrist, SJ (2013) The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles. J. Cell Biol. 202, 667-83.

Fogeron ML, Müller H, Schade S, Dreher F, Lehmann V, Kühnel A, Scholz AK, Kashofer K, Zerck A, Fauler B, Lurz R, Herwig R, Zatloukal K, Lehrach H, Gobom J, Nordhoff E and Lange BM (2013) LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells. Nat Commun 4, 1531. doi: 10.1038/ncomms2517.

Donczew R, Weigel C, Lurz R, Zakrzewska-Czerwinska J, Zawilak-Pawlik A (2012) Helicobacter pylori oriC - the first bipartite origin of chromosome replication in Gram-negative bacteria. Nucleic Acids Res 40, 9647-60.

Kaden D, Harmeier A, Weise C, Munter LM, Althoff V, Rost BR, Hildebrand PW, Schmitz D, Schaefer M, Lurz R, Skodda S, Yamamoto R, Arlt S, Finckh U, Multhaup G (2012) Novel APP/Aβ mutation K16N produces highly toxic heteromeric Aβ oligomers. EMBO Mol Med 4, 647-59.

Solano-Collado V, Lurz R, Espinosa M, Bravo A (2013) The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA. Nucleic Acids Res 41, 6975-91.

Evrin C, Fernández-Cid A, Zech J, Herrera MC, Riera A, Clarke P, Brill S, Lurz R, Speck C (2013) In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization. Nucleic Acids Res 41, 3162-72.

 

2012

Ramrath DJF, Yamamoto H, Rother K, Wittek D, Pech M, Mielke T, Loerke J, Scheerer P, Ivanov P, Teraoka Y, Shpanchenko O, Nierhaus KH and Spahn CMT (2012) The complex of tmRNA–SmpB and EF-G on translocating ribosomes. Nature 485, 526-529.

Maisel T, Joseph S, Mielke T, Bürger J, Schwarzinger S and Meyer O (2012) The CoxD Protein, a Novel AAA+ ATPase Involved in Metal Cluster Assembly: Hydrolysis of Nucleotide-Triphosphates and Oligomerization. PLoS One 7(10): e47424.

Kim KP, Born Y, Lurz R, Eichenseher F, Zimmer M, Loessner MJ, Klumpp J (2012) Inducible Clostridium perfringens bacteriophages ΦS9 and ΦS63: Different genome structures and a fully functional sigK intervening element. Bacteriophage 2, 89-97.

Sudheer S, Bhushan R, Fauler B, Lehrach H and Adjaye J (2012) FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast. Stem Cells Dev 21, 2987-3000.

Vinga I, Baptista C, Auzat I, Petipas I, Lurz R, Tavares P, Santos MA, São-José C (2012) Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor binding and trigger of phage DNA ejection. Mol Microbiol 83, 289-303.

Donczew R, Weigel C, Lurz R, Zakrzewska-Czerwinska J, Zawilak-Pawlik A (2012) Helicobacter pylori oriC - the first bipartite origin of chromosome replication in Gram-negative bacteria. Nucleic Acids Res 40, 9647-60.

Kaden D, Harmeier A, Weise C, Munter LM, Althoff V, Rost BR, Hildebrand PW, Schmitz D, Schaefer M, Lurz R, Skodda S, Yamamoto R, Arlt S, Finckh U, Multhaup G (2012) Novel APP/Aβ mutation K16N produces highly toxic heteromeric Aβ oligomers. EMBO Mol Med 4, 647-59.

Jakutytė L, Lurz R, Baptista C, Carballido-Lopez R, São-José C, Tavares P, Daugelavičius R (2012) First steps of bacteriophage SPP1 entry into Bacillus subtilis. Virology 422, 425-34.

 

2011

Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC and Spahn CMT (2011) Structure and dynamics of the mammalian ribosomal pretranslocation complex. Mol Cell 44, 214-24.

Prigione A, Hossini AM, Lichtner B, Serin A, Fauler B, Megges M, Lurz R, Lehrach H, Makrantonika E, Zouboulis CC and Adjaye J (2011) Mitochondrial-Associated Cell Death Mechanisms Are Reset to an Embryonic-Like State in Aged Donor-Derived iPS Cells Harboring Chromosomal Aberrations. PLoS ONE 6(11): e27352. doi:10.1371/journal.pone.0027352.

Becker T, Armache JP, Jarasch A, Anger AM, Villa E, Sieber H, Motaal BA, Mielke T, Berninghausen O and Beckmann R (2011) Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome. Nat Struct Mol Biol. 18, 715-20.

Bieschke J, Herbst M, Wiglenda T, Friedrich RP, Boeddrich A, Schiele F, Kleckers D, Lopez del Amo JM, Grüning BA, Wang Q, Schmidt MR, Lurz R, Anwyl R, Schnoegl S, Fändrich M, Frank RF, Reif B, Günther S, Walsh DM, Wanker EE (2011) Small-molecule conversion of toxic oligomers to nontoxic β-sheet-rich amyloid fibrils. Nat Chem Biol 8, 93-101.

Czeko E, Seizl M, Augsberger C, Mielke T and Cramer P (2011) Iwr1 Directs RNA Polymerase II Nuclear Import. Mol. Cell 42, 261–266.

Aramayo R, Sherman MB, Brownless K, Lurz R, Okorokov AL, Orlova EV (2011) Quaternary structure of the specific p53-DNA complex reveals the mechanism of p53 mutant dominance. Nucleic Acids Res 39, 8960-71.

Frauenfeld J, Gumbart J, van der Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K and Beckmann R (2011) Cryo-EM structure of the ribosome–SecYE complex in the membrane environment. Nat Struct Mol Biol. 18, 614-621.

Muhs M, Yamamoto H, Ismer J, Takaku H, Nashimoto M, Uchiumi T, Nakashima N, Mielke T, Hildebrand PW, Nierhaus KH and Spahn,CMT (2011) Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit. Nucleic Acids Res. 39, 5264-75.

Bhushan S, Hoffman T, Seidelt B, Mielke T, Berninghausen O, Wilson DN and Beckmann R (2011) SecM-Stalled Ribosomes Adopt an Altered Geometry at the Peptidyl Transferase Center. PLoS Biol 9(1): e1000581. doi:10.1371/journal.pbio.1000581.

 

2010

Ratje AH, Loerke J, Mikolajka A, Brünner M, Hildebrand PW, Starosta A, Dönhöfer A, Connell SR, Fucini P, Mielke T, Whitford PC, Onuchic JN, Yu Y, Sanbonmatsu KY, Hartmann RK, Penczek PA, Wilson DN and Spahn CMT (2010) Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites. Nature 468, 713-716.

Prigione A, Fauler B, Lurz R, Lehrach H and Adjaye J (2010) The Senescence-Related Mitochondrial/Oxidative Stress Pathway is Repressed in Human Induced Pluripotent Stem Cells. Stem Cells 28, 721–733.

Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Söding J, Westhof E, Wilson DN and Beckmann R (2010) Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci USA 107, 19748-19753.

Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Söding J, Westhof E, Wilson DN and Beckmann R (2010) Localization of eukaryote-specific ribosomal proteins in a 5.5 Å cryo-EM map of the 80S eukaryotic ribosome. Proc. Natl. Acad. Sci. USA 107, 19754-19759.

Bhushan S, Meyer H, Starosta AL, Becker T, Mielke T, Berninghausen O, Sattler M, Wilson DN and Beckmann R (2010) Structural basis for translational stalling by human cytomegalovirus (hCMV) and fungal arginine attenuator peptide (AAP). Mol Cell 40, 138-146.

Gartmann M, Blau M, Armache JP, Mielke T, Topf M and Beckmann R (2010) Mechanism of eIF6 mediated inhibition of ribosomal subunit joining. J Biol Chem. 285, 14848-14851.

Bhushan S, Gartmann M, Halic M, Armache JP, Jarasch A, Mielke T, Berninghausen O, Wilson DN and Beckmann R (2010) a-helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel. Nature Structural & Molecular Biology 17, 313-7.

Liu C, Young AL, Starling-Windhof A, Bracher A, Saschenbrecker S, Vasudeva Rao B, Vasudeva Rao K, Berninghausen O, Mielke T, Hartl FU, Beckmann R and Hayer-Hartl M (2010) Coupled chaperone action in folding and assembly of hexadecameric Rubisco. Nature 463, 197-202.

 

2009

Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart JC, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC and Beckmann R (2009) Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome, Science 326, 1369-1373.

Seidelt B, Innis CA, Wilson DN, Gartmann M, Armache JP, Trabuco LG, Becker T, Mielke T, Schulten K, Steitz TA and Beckmann R (2009) Structural insight into nascent chain-mediated translational stalling, Science 326, 1412-1415.

Schuette JC, Murphy FV 4th, Kelley AC, Giesebrecht J, Connell SR, Loerke J, Mielke T, Zhang W, Penczek PA, Ramakrishnan V and Spahn CMT (2009) GTPase activation of elongation factor EF-Tu by the ribosome during decoding. EMBO J. 28, 755–765.

 

2008

Connell SR, Topf M, Qin Y, Wilson DN, Mielke T, Fucini P, Nierhaus KH and Spahn CMT (2008) A novel tRNA-intermediate revealed on the ribosome during EF4 (LepA)-mediated back-translocation. Nature Structural & Molecular Biology 15, 910-915.

Chen J, Sachse C, Xu C, Mielke T, Spahn CMT and Grigorieff N (2008) A Dose-Rate Effect in Single-Particle Electron Microscopy. J. Struc. Biol. 161, 92-100.

 

2007

Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R and Cramer P (2007) Functional architecture of RNA polymerase I. Cell 131, 1260-1272.

Connnell SR, Takemoto C, Wilson DN, Wang H, Murayama K, Terada T, Shirouzu M, Rost M, Schüler M, Giesebrecht J, Dabrowski M, Mielke T, Fucini PM, Yokoyama S and Spahn CMT (2007) Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors. Molecular Cell 25, 751-764.

 

2006

Schüler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E and Spahn CMT (2006) Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nature Structural & Molecular Biology 13, 1092-1096.

Halic M, Blau M, Becker T, Mielke T, Pool MR, Wild K, Sinning I and Beckmann R (2006) Following the signal sequence from ribosomal tunnel exit to signal recognition particle. Nature 444, 507-511.

Andersen CBF, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Petersen JS, Spahn CMT, Goss Kinzy T, Andersen GR and Beckmann R (2006) Structure of eEF3 and the mechanism of transfer RNA release from the E-site. Nature 443, 663-668.

Halic M, Gartmann M, Schlenker O, Mielke T, Pool MR, Sinning I and Beckmann R (2006) Signal recognition particle receptor exposes the ribosomal translocon binding site. Science 312, 745-747.

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