List  of Publications

2017

Said N, Krupp F, Anedchenko E, Santos K, Dybkov O, Huang Y-H, Lee C-T, Loll B, Behrmann E, Bürger J, Mielke T, Loerke J, Urlaub H, Spahn CM, Weber G, Wahl, M (2017) Structural basis for λN-dependent processive transcription antitermination. Nature Microbiology, accepted

Lorenz C, Lesimple P, Bukowiecki R, Zink A, Inak G, Mlody B, Singh M, Semtner M, Mah N, Auré K, Leong M, Zabiegalov O, Lyras EM, Pfiffer V, Fauler B, Eichhorst J, Wiesner B, Huebner N, Priller J, Mielke T, Meierhofer D, Izsvák Z, Meier JC, Bouillaud F, Adjaye J, Schuelke M, Wanker EE, Lombès A, Prigione A (2017) Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders. Cell Stem Cell. 2017 Jan 25. pii: S1934-5909(16)30469-6. doi: 10.1016/j.stem.2016.12.013. [Epub ahead of print]

2016

Hilal T, Yamamoto H, Loerke J, Bürger J, Mielke T, Spahn CM (2016) Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution. Nat Commun. 2016 Dec 20;7:13521. doi: 10.1038/ncomms13521.

Arumughan A, Roske Y, Barth C, Forero LL, Bravo-Rodriguez K, Redel A, Kostova S, McShane E, Opitz R, Faelber K, Rau K, Mielke T, Daumke O, Selbach M, Sanchez-Garcia E, Rocks O, Panáková D, Heinemann U, Wanker EE (2016) Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. Nat. Commun. 7:13047.

Jaworski P, Donczew R, Mielke T, Thiel M, Oldziej S, Weigel C, Zawilak-Pawlik A (2016) Unique and Universal Features of Epsilonproteobacterial Origins of Chromosome Replication and DnaA-DnaA Box Interactions. Front Microbiol. 7:1555

Gupta VK, Pech U, Bhukel A, Fulterer A, Ender A, Mauermann SF, Andlauer TF, Antwi-Adjei E, Beuschel C, Thriene K, Maglione M, Quentin C, Bushow R, Schwärzel M, Mielke T, Madeo F, Dengjel J, Fiala A, Sigrist SJ (2016) Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release. PLoS Biol. 14(9):e1002563. doi: 10.1371/journal.pbio.1002563

Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke L, Ismer J, Hildebrand PW, Scheerer P, Bürger J, Mielke T, Spahn CMT (2016) Structures of ribosome-bound initiation factor 2 reveal mechanism of subunit association. Sci. Adv. 2(3):e1501502. doi: 10.1126/sciadv.1501502

2015

Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Bürger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT (2015) Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA. EMBO J. 34, 3042-58.

Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Bürger J, Mielke T, Scheerer P, Spahn CMT (2015) Structural snapshots of actively translating human ribosomes. Cell 161, 845-57.

Chaban Y, Lurz R, Brasilès S, Cornilleau C, Karreman M, Zinn-Justin S, Tavares P, Orlova EV (2015) Structural rearrangements in the phage head-to-tail interface during assembly and infection. Proc Natl Acad Sci U S A 112, 7009-14.

van der Sluis EO, Bauerschmitt H, Becker T, Mielke T, Frauenfeld J, Berninghausen O, Neupert W, Herrmann JM, Beckmann R (2015) Parallel Structural Evolution of Mitochondrial Ribosomes and OXPHOS Complexes. Genome Biol Evol 7, 1235-51.

Bielmann R, Habann M, Eugster MR, Lurz R, Calendar R, Klumpp J, Loessner MJ (2015) Receptor binding proteins of Listeria monocytogenes bacteriophages A118 and P35 recognize serovar-specific teichoic acids. Virology 477, 110-8.

Hammerl JA, Roschanski N, Lurz R, Johne R, Lanka E, Hertwig S (2015) The Molecular Switch of Telomere Phages: High Binding Specificity of the PY54 Cro Lytic Repressor to a Single Operator Site. Viruses 7, 2771-93.

Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM (2015) Cryo-EM of ribosomal 80S complexes with termination factors reveals the translocated Cricket Paralysis Virus IRES. Mol Cell. 57, 422-32.

2014

Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Bürger J, Mielke T and Spahn CMT (2014) Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA. Nat Struct Mol Biol. 21, 721-7.

Röthlein C, Miettinen MS, Borwankar T, Bürger J, Mielke T, Kumke MU and Ignatova Z (2014) Architecture of polyglutamine-containing fibrils from time-resolved fluorescence decay. J Biol Chem. 289, 26817-28.

Budkevich TV, Giesebrecht J, Behrmann E, Loerke J, Ramrath DJF, Mielke T, Ismer J, Hildebrand PW, Tung CS, Nierhaus KH, Sanbonmatsu KY and Spahn CMT (2014) Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement. Cell 158, 121-31.

Donczew R, Mielke T, Jaworski P, Zakrzewska-Czerwińska J and Zawilak-Pawlik A (2014) Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions. J Mol Biol 426, 2769-82.

Barucker C, Harmeier A, Weiske J, Fauler B, Albring KF, Prokop S, Hildebrand P, Lurz R, Heppner FL, Huber O, Multhaup G. (2014) Nuclear translocation uncovers the amyloid peptide Aβ42 as a regulator of gene transcription. J Biol Chem 289, 20182-91.

Shah T, Hegde BG, Morén B, Behrmann E, Mielke T, Moenke G, Spahn CMT, Lundmark R, Daumke O and Langen R (2014) Structural insights into membrane interaction and caveolar targeting of dynamin-like EHD2. Structure 22, 409-20.

Auzat I, Petitpas I, Lurz R, Weise F, Tavares P (2014) A touch of glue to complete bacteriophage assembly: the tail-to-head joining protein (THJP) family. Mol Microbiol 91, 1164-78.

Evrin C, Fernández-Cid A, Riera A, Zech J, Clarke P, Herrera MC, Tognetti S, Lurz R, Speck C (2014) The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res 42, 2257-69.

Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, Renouard M, Huet A, Decottignies P, Davidson AR, Breyton C, Boulanger P (2014) Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components. J Virol 88, 1162-74.

Flayhan A, Vellieux FM, Lurz R, Maury O, Contreras-Martel C, Girard E, Boulanger P, Breyton C (2014) Crystal structure of pb9, the distal tail protein of bacteriophage T5: a conserved structural motif among all siphophages. J Virol 88, 820-8.

2013

Ramrath DJF, Lancaster L, Sprink T, Mielke T, Loerke J, Noller HF and Spahn CMT (2013) Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation. Proc Natl Acad Sci USA 110, 20964-20969.

Klatt S, Hartl D, Fauler B, Gagoski D, Castro-Obrego S and Konthur Z (2013) Generation and Characterization of a Leishmania tarentolae Strain for Site-Directed in Vivo Biotinylation of Recombinant Proteins. J Proteome Res 12, 5512-5519.

Matkovic T, Siebert M, Knoche E, Depner H, Mertel S, Owald D, Schmidt M, Thomas U, Sickmann A, Kamin D, Hell SW, Bürger J, Hollmann C, Mielke T, Wichmann C and Sigrist, SJ (2013) The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles. J. Cell Biol. 202, 667-83.

Fogeron ML, Müller H, Schade S, Dreher F, Lehmann V, Kühnel A, Scholz AK, Kashofer K, Zerck A, Fauler B, Lurz R, Herwig R, Zatloukal K, Lehrach H, Gobom J, Nordhoff E and Lange BM (2013) LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells. Nat Commun 4, 1531. doi: 10.1038/ncomms2517.

Donczew R, Weigel C, Lurz R, Zakrzewska-Czerwinska J, Zawilak-Pawlik A (2012) Helicobacter pylori oriC - the first bipartite origin of chromosome replication in Gram-negative bacteria. Nucleic Acids Res 40, 9647-60.

Kaden D, Harmeier A, Weise C, Munter LM, Althoff V, Rost BR, Hildebrand PW, Schmitz D, Schaefer M, Lurz R, Skodda S, Yamamoto R, Arlt S, Finckh U, Multhaup G (2012) Novel APP/Aβ mutation K16N produces highly toxic heteromeric Aβ oligomers. EMBO Mol Med 4, 647-59.

Solano-Collado V, Lurz R, Espinosa M, Bravo A (2013) The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA. Nucleic Acids Res 41, 6975-91.

Evrin C, Fernández-Cid A, Zech J, Herrera MC, Riera A, Clarke P, Brill S, Lurz R, Speck C (2013) In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization. Nucleic Acids Res 41, 3162-72.

2012

Ramrath DJF, Yamamoto H, Rother K, Wittek D, Pech M, Mielke T, Loerke J, Scheerer P, Ivanov P, Teraoka Y, Shpanchenko O, Nierhaus KH and Spahn CMT (2012) The complex of tmRNA–SmpB and EF-G on translocating ribosomes. Nature 485, 526-529.

Maisel T, Joseph S, Mielke T, Bürger J, Schwarzinger S and Meyer O (2012) The CoxD Protein, a Novel AAA+ ATPase Involved in Metal Cluster Assembly: Hydrolysis of Nucleotide-Triphosphates and Oligomerization. PLoS One 7(10): e47424.

Kim KP, Born Y, Lurz R, Eichenseher F, Zimmer M, Loessner MJ, Klumpp J (2012) Inducible Clostridium perfringens bacteriophages ΦS9 and ΦS63: Different genome structures and a fully functional sigK intervening element. Bacteriophage 2, 89-97.

Sudheer S, Bhushan R, Fauler B, Lehrach H and Adjaye J (2012) FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast. Stem Cells Dev 21, 2987-3000.

Vinga I, Baptista C, Auzat I, Petipas I, Lurz R, Tavares P, Santos MA, São-José C (2012) Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor binding and trigger of phage DNA ejection. Mol Microbiol 83, 289-303.

Donczew R, Weigel C, Lurz R, Zakrzewska-Czerwinska J, Zawilak-Pawlik A (2012) Helicobacter pylori oriC - the first bipartite origin of chromosome replication in Gram-negative bacteria. Nucleic Acids Res 40, 9647-60.

Kaden D, Harmeier A, Weise C, Munter LM, Althoff V, Rost BR, Hildebrand PW, Schmitz D, Schaefer M, Lurz R, Skodda S, Yamamoto R, Arlt S, Finckh U, Multhaup G (2012) Novel APP/Aβ mutation K16N produces highly toxic heteromeric Aβ oligomers. EMBO Mol Med 4, 647-59.

Jakutytė L, Lurz R, Baptista C, Carballido-Lopez R, São-José C, Tavares P, Daugelavičius R (2012) First steps of bacteriophage SPP1 entry into Bacillus subtilis. Virology 422, 425-34.

2011

Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC and Spahn CMT (2011) Structure and dynamics of the mammalian ribosomal pretranslocation complex. Mol Cell 44, 214-24.

Prigione A, Hossini AM, Lichtner B, Serin A, Fauler B, Megges M, Lurz R, Lehrach H, Makrantonika E, Zouboulis CC and Adjaye J (2011) Mitochondrial-Associated Cell Death Mechanisms Are Reset to an Embryonic-Like State in Aged Donor-Derived iPS Cells Harboring Chromosomal Aberrations. PLoS ONE 6(11): e27352. doi:10.1371/journal.pone.0027352.

Becker T, Armache JP, Jarasch A, Anger AM, Villa E, Sieber H, Motaal BA, Mielke T, Berninghausen O and Beckmann R (2011) Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome. Nat Struct Mol Biol. 18, 715-20.

Bieschke J, Herbst M, Wiglenda T, Friedrich RP, Boeddrich A, Schiele F, Kleckers D, Lopez del Amo JM, Grüning BA, Wang Q, Schmidt MR, Lurz R, Anwyl R, Schnoegl S, Fändrich M, Frank RF, Reif B, Günther S, Walsh DM, Wanker EE (2011) Small-molecule conversion of toxic oligomers to nontoxic β-sheet-rich amyloid fibrils. Nat Chem Biol 8, 93-101.

Czeko E, Seizl M, Augsberger C, Mielke T and Cramer P (2011) Iwr1 Directs RNA Polymerase II Nuclear Import. Mol. Cell 42, 261–266.

Aramayo R, Sherman MB, Brownless K, Lurz R, Okorokov AL, Orlova EV (2011) Quaternary structure of the specific p53-DNA complex reveals the mechanism of p53 mutant dominance. Nucleic Acids Res 39, 8960-71.

Frauenfeld J, Gumbart J, van der Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K and Beckmann R (2011) Cryo-EM structure of the ribosome–SecYE complex in the membrane environment. Nat Struct Mol Biol. 18, 614-621.

Muhs M, Yamamoto H, Ismer J, Takaku H, Nashimoto M, Uchiumi T, Nakashima N, Mielke T, Hildebrand PW, Nierhaus KH and Spahn,CMT (2011) Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit. Nucleic Acids Res. 39, 5264-75.

Bhushan S, Hoffman T, Seidelt B, Mielke T, Berninghausen O, Wilson DN and Beckmann R (2011) SecM-Stalled Ribosomes Adopt an Altered Geometry at the Peptidyl Transferase Center. PLoS Biol 9(1): e1000581. doi:10.1371/journal.pbio.1000581.

2010

Ratje AH, Loerke J, Mikolajka A, Brünner M, Hildebrand PW, Starosta A, Dönhöfer A, Connell SR, Fucini P, Mielke T, Whitford PC, Onuchic JN, Yu Y, Sanbonmatsu KY, Hartmann RK, Penczek PA, Wilson DN and Spahn CMT (2010) Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites. Nature 468, 713-716.

Prigione A, Fauler B, Lurz R, Lehrach H and Adjaye J (2010) The Senescence-Related Mitochondrial/Oxidative Stress Pathway is Repressed in Human Induced Pluripotent Stem Cells. Stem Cells 28, 721–733.

Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Söding J, Westhof E, Wilson DN and Beckmann R (2010) Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci USA 107, 19748-19753.

Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Söding J, Westhof E, Wilson DN and Beckmann R (2010) Localization of eukaryote-specific ribosomal proteins in a 5.5 Å cryo-EM map of the 80S eukaryotic ribosome. Proc. Natl. Acad. Sci. USA 107, 19754-19759.

Bhushan S, Meyer H, Starosta AL, Becker T, Mielke T, Berninghausen O, Sattler M, Wilson DN and Beckmann R (2010) Structural basis for translational stalling by human cytomegalovirus (hCMV) and fungal arginine attenuator peptide (AAP). Mol Cell 40, 138-146.

Gartmann M, Blau M, Armache JP, Mielke T, Topf M and Beckmann R (2010) Mechanism of eIF6 mediated inhibition of ribosomal subunit joining. J Biol Chem. 285, 14848-14851.

Bhushan S, Gartmann M, Halic M, Armache JP, Jarasch A, Mielke T, Berninghausen O, Wilson DN and Beckmann R (2010) a-helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel. Nature Structural & Molecular Biology 17, 313-7.

Liu C, Young AL, Starling-Windhof A, Bracher A, Saschenbrecker S, Vasudeva Rao B, Vasudeva Rao K, Berninghausen O, Mielke T, Hartl FU, Beckmann R and Hayer-Hartl M (2010) Coupled chaperone action in folding and assembly of hexadecameric Rubisco. Nature 463, 197-202.

2009

Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart JC, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon EC and Beckmann R (2009) Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome, Science 326, 1369-1373.

Seidelt B, Innis CA, Wilson DN, Gartmann M, Armache JP, Trabuco LG, Becker T, Mielke T, Schulten K, Steitz TA and Beckmann R (2009) Structural insight into nascent chain-mediated translational stalling, Science 326, 1412-1415.

Schuette JC, Murphy FV 4th, Kelley AC, Giesebrecht J, Connell SR, Loerke J, Mielke T, Zhang W, Penczek PA, Ramakrishnan V and Spahn CMT (2009) GTPase activation of elongation factor EF-Tu by the ribosome during decoding. EMBO J. 28, 755–765.

2008

Connell SR, Topf M, Qin Y, Wilson DN, Mielke T, Fucini P, Nierhaus KH and Spahn CMT (2008) A novel tRNA-intermediate revealed on the ribosome during EF4 (LepA)-mediated back-translocation. Nature Structural & Molecular Biology 15, 910-915.

Chen J, Sachse C, Xu C, Mielke T, Spahn CMT and Grigorieff N (2008) A Dose-Rate Effect in Single-Particle Electron Microscopy. J. Struc. Biol. 161, 92-100.

2007

Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R and Cramer P (2007) Functional architecture of RNA polymerase I. Cell 131, 1260-1272.

Connnell SR, Takemoto C, Wilson DN, Wang H, Murayama K, Terada T, Shirouzu M, Rost M, Schüler M, Giesebrecht J, Dabrowski M, Mielke T, Fucini PM, Yokoyama S and Spahn CMT (2007) Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors. Molecular Cell 25, 751-764.

2006

Schüler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E and Spahn CMT (2006) Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nature Structural & Molecular Biology 13, 1092-1096.

Halic M, Blau M, Becker T, Mielke T, Pool MR, Wild K, Sinning I and Beckmann R (2006) Following the signal sequence from ribosomal tunnel exit to signal recognition particle. Nature 444, 507-511.

Andersen CBF, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Petersen JS, Spahn CMT, Goss Kinzy T, Andersen GR and Beckmann R (2006) Structure of eEF3 and the mechanism of transfer RNA release from the E-site. Nature 443, 663-668.

Halic M, Gartmann M, Schlenker O, Mielke T, Pool MR, Sinning I and Beckmann R (2006) Signal recognition particle receptor exposes the ribosomal translocon binding site. Science 312, 745-747.

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