Publications of Thorsten Mielke

Hiepen, C.; Benamar, M.; Barrasa-Fano, J.; Condor, M.; Ilhan, M.; Münch, J.; Hastar, N.; Kerkhoff, Y.; Harms, G. S.; Mielke, T. et al.; Koenig, B.; Block, S.; Rocks, O.; Abdelilah-Seyfried, S.; Van Oosterwyck , H.; Knaus, P.: Endothelial tip-cell position, filopodia formation and biomechanics require BMPR2 expression and signaling. Communications Biology 8 (1), Article 21 (2025)

Wiechert, F.; Unbehaun, A.; Sprink, T.; Seibel, H.; Bürger, J.; Loerke, J.; Mielke, T.; Diebolder , C. A.; Schacherl , M.; Spahn, C. M. T.: Visualizing the modification landscape of the human 60S ribosomal subunit at close to atomic resolution. Nucleic Acids Research 53 (1), Article gkae1191 (2025)

Lützkendorf, J.; Matkovic-Rachid, T.; Liu, S.; Götz, T.; Gao, L.; Turrel, O.; Maglione, M.; Grieger, M.; Putignano, S.; Ramesh, N. et al.; Ghelani, T.; Neumann, A.; Gimber, N.; Schmoranzer, J.; Stawrakakis, A.; Brence, B.; Baum, D.; Ludwig, K.; Heine, M.; Mielke, T.; Liu, F.; Walter, A. M.; Wahl, M. C.; Sigrist, S. J.: Blobby is a synaptic active zone assembly protein required for memory in Drosophila. Nature Communications 16 (1), Article 271 (2025)

Auzat, I.; Ouldali, M.; Jacquet, E.; Fauler, B.; Mielke, T.; Tavares, P.: Dual function of a highly conserved bacteriophage tail completion protein essential for bacteriophage infectivity. Communications Biology 7 (1), Article 590 (2024)

Flügel, T.; Schacherl, M.; Unbehaun, A.; Schroeer, B.; Dabrowski, M.; Bürger, J.; Mielke, T.; Sprink, T.; Diebolder, C. A.; Guillén Schlippe , Y. V. et al.; Spahn , C. M. T.: Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM. Nature Communications 15 (1), Article 1756 (2024)

van der Weijden, V. A.; Stötzel, M.; Iyer, D. P.; Fauler, B.; Gralinska, E.; Shahraz, M.; Meierhofer, D.; Vingron, M.; Rulands, S.; Alexandrov, T. et al.; Mielke, T.; Bulut-Karslioglu, A.: FOXO1-mediated lipid metabolism maintains mammalian embryos in dormancy. Nature Cell Biology 26 (2), pp. 181 - 193 (2024)

Qin, B.; Lauer, S. M.; Balke, A.; Vieira-Vieira, C. H.; Bürger, J.; Mielke, T.; Selbach, M.; Scheerer, P.; Spahn, C. M. T.; Nikolay, R.: Cryo-EM captures early ribosome assembly in action. Nature Communications 14 (1), Article 898 (2023)

Le-Trilling, V. T. K.; Banchenko, S.; Paydar, D.; Leipe, P. M.; Binting, L.; Lauer, S.; Graziadei, A.; Klingen, R.; Gotthold, C.; Bürger, J. et al.; Bracht, T.; Sitek, B.; Lebbink, R. J.; Malyshkina, A.; Mielke, T.; Rappsilber, J.; Spahn, C. M. T.; Voigt, S.; Trilling, M.; Schwefel, D.: Structural mechanism of CRL4-instructed STAT2 degradation via a novel cytomegaloviral DCAF receptor. EMBO Journal 42 (5), e112351 (2023)

Landshammer, A.; Bolondi, A.; Kretzmer, H.; Much, C.; Buschow, R.; Rose, A.; Wu, H.-J.; Mackowiak, S. D.; Braendl, B.; Gießelmann, P. et al.; Tornisiello, R.; Mohan Parsi, K.; Huey, J.; Mielke, T.; Meierhofer, D.; Maehr, R.; Hnisz, D.; Michor, F.; Rinn, J. L.; Meissner, A.: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation. eLife 12, Article e83077 (2023)

Smirnova, J.; Loerke, J.; Kleinau, G.; Schmidt, A.; Bürger, J.; Meyer, E. H.; Mielke, T.; Scheerer, P.; Bock, R.; Spahn, C. M. T. et al.; Zoschke, R.: Structure of the actively translating plant 80S ribosome at 2.2 Å resolution. Nature Plants 9 (6), pp. 987 - 1000 (2023)

Harnett, D.; Ambrozkiewicz, M. C.; Zinnall, U.; Rusanova, A.; Borisova, E.; Drescher, A.; Couce Iglesias, M.; Dannenberg, R.; Imami, K.; Münster-Wandowski,, A. et al.; Fauler, B.; Mielke, T.; Selbach, M.; Landthaler, M.; Spahn, C. M. T.; Tarabykin, V.; Ohler, U.; Kraushar, M. L.: A critical period of translational control during brain development at codon resolution. Nature Structural Molecular Biology 29 (12), pp. 1277 - 1290 (2022)

Kornak, U.; Saha, N.; Keren, B.; Neumann, A.; Taylor Tavares, A. L.; Piard, J.; Kopp, J.; Rodrigues Alves, J. G.; de los Santos, M. R.; El Choubassi, N. et al.; Ehmke, N.; Jäger, M.; Spielmann, M.; Pantel, J. T.; Lejeune, E.; Fauler, B.; Mielke, T.; Hecht, J.; Meierhofer, D.; Strom, T. M.; Laugel, V.; Brice, A.; Mundlos, S.; Bertoli-Avella, A.; Bauer, P.; Heyd, F.; Boute, O.; Dupont, J.; Depienne, C.; Van Maldergem, L.; Fischer-Zirnsak, B.: Alternative splicing of BUD13 determines the severity of a developmental disorder with lipodystrophy and progeroid features. GENETICS IN MEDICINE 24 (9), pp. 1927 - 1940 (2022)

Hilal, T.; Killam, B. Y.; Grozdanović, M.; Dobosz-Bartoszek, M.; Loerke, J.; Bürger, J.; Mielke, T.; Copeland, P. R.; Simonović, M.; Spahn, C. M. T.: Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon. Science 376, pp. 1338 - 1343 (2022)

Hochmair, J.; Exner, C.; Franck, M.; Dominguez-Baquero, A.; Diez, L.; Brognaro, H.; Kraushar, M. L.; Mielke, T.; Radbruch, H.; Kaniyappan, S. et al.; Falke, S.; Mandelkow, E.; Betzel, C.; Wegmann, S.: Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates. The EMBO Journal 41 (11), e108882 (2022)

Heyder, N. A.; Kleinau, G.; Speck, D.; Schmidt, A.; Paisdzior, S.; Szczepek, M.; Bauer, B.; Koch, A.; Gallandi, M.; Kwiatkowski, D. et al.; Bürger, J.; Mielke, T.; Beck-Sickinger, A. G.; Hildebrand, P. W.; Spahn, C. M. T.; Hilger, D.; Schacherl, M.; Biebermann, H.; Hilal, T.; Kühnen, P.; Kobilka, B. K.; Scheerer, P.: Structures of active melanocortin-4 receptor−Gs-protein complexes with NDP-α-MSH and setmelanotide. Cell Research 31 (11), pp. 1176 - 1189 (2021)

Banchenko, S.; Krupp, F.; Gotthold, C.; Bürger, J.; Graziadei, A.; O'Reilly, F. J.; Sinn, L.; Ruda, O.; Rappsilber, J.; Spahn , C. M. T. et al.; Mielke, T.; Taylor, I. A. ..; Schwefel, D.: Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses. PLoS Pathogens 17 (8), e1009775 (2021)

Labarde, A.; Jakutyte, L.; Billaudeau, C.; Fauler, B.; López-Sanz, M.; Ponien, P.; Jacquet, E.; Mielke, T.; Ayora, S.; Carballido-López, R. et al.; Tavares, P.: Temporal compartmentalization of viral infection in bacterial cells. Proceedings of the National Academy of Sciences of the United States of America 118 (28), e2018297118 (2021)

Haggerty, C.; Kretzmer, H.; Riemenschneider, C.; Sampath Kumar, A.; Mattei, A. L.; Bailly, N.; Gottfreund, J.; Giesselmann, P.; Weigert, R.; Brändl, B. et al.; Giehr, P.; Buschow, R.; Galonska, C.; von Meyenn, F.; Pappalardi, M. B.; McCabe, M. T.; Wittler, L.; Giesecke-Thiel, C.; Mielke, T.; Meierhofer, D.; Timmermann, B.; Müller, F.-J.; Walter, J.; Meissner, A.: Dnmt1 has de novo activity targeted to transposable elements. Nature Structural and Molecular Biology 28 (7), pp. 594 - 603 (2021)

Jaworski, P.; Zyla-Uklejewicz, D.; Nowaczyk-Cieszewska, M.; Donczew, R.; Mielke, T.; Weigel, C.; Zawilak-Pawlik, A.: Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly. International Journal of Molecular Sciences 22 (12), 6643 (2021)

Pooryasin, A.; Maglione, M.; Schubert, M.; Matkovic-Rachid, T.; Hasheminasab, S.-M.; Pech, U.; Fiala, A.; Mielke, T.; Sigrist, S. J.: Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila. Nature Communications (12), 1932 (2021)