Nanocourse: Cancer Genomics

“ Portraits of a tumor: cancer cells and their microenvironment or building a new world in a tissue”

Nanocourses consist of lectures followed by the opportunity to gain hands-on experience.

Please register for this course by emailing imprs-bac@molgen.mpg.de.

Schedule

10:00 – 10:45 Introduction to Cancer Genomics (Marie-Laure Yaspo) (SR4)

10:45 – 11:00 Coffee break

11:00 – 11:45 Lecture by Prof. Christian Conrad BIH. Charité: “Single cell sequencing and imaging” (SR4)

11:45 – 12:15 Time for discussion (SR4)

12:15 – 13:00 Lunch break

13:00 – 14:00 Introduction to Imaging mass cytometry (IMC), Image analysis (Anika Rettig) (SR1)

14:00 – 15:00 Visit to the lab and Hyperion instrument (Alexander Kovacsovics) (Yaspo's lab)

15:00 – 16:30 Hands on Hyperion data and image analysis, and open discussions (PC room)

10:00 – 11:00 Lecture: Transcriptomics, eRNAs and gene regulation networks in cancer (Marie-Laure Yaspo, Begüm Özemek) (SR1)

11:00 – 12:30 Journal club (the group to pick 1-3 papers to discuss) (SR1)

L. Kuett et al. Three-dimensional imaging mass cytometry for highly multiplexed molecular and cellular mapping of tissues and the tumor microenvironment. Nature Cancer PMID: 35121992
M. Schütte et al. Molecular dissection of colorectal cancer in pre-clinical models identifies biomarkers predicting sensitivity to EGFR inhibitors. Nat Comms. PMID: 28186126
Karras et al. A cellular hierarchy in melanoma uncouples growth and metastasis. Nature. PMID: 36131018

12:30 – 13:30 Lunch break

13:30 – 14:30 Technologies to decipher gene regulation networks (ChiPseq, Cut&Run) (Ozan Otas) (SR1)

14:30 – 14:45 Coffee break

14:45 – 16:00 Data hands-on with ChipSeq and Cut&Run (Wanwan Ge) (PC room)

16:00 – 17:00 CROPseq technologies and functional networks (Mathurin Dorel) (SR1)