Sequencing Core Facility
New sequencing technologies have revolutionized genomic research over the last decade. Today, they enable researchers to investigate (epi)genomes and transcriptomes at an unexampled depth and level of detail.
Founded in 2007 the Next Generation Sequencing (NGS) Group at the Max Planck Institute for molecular Genetics is a central unit which provides its service to all research groups of the institute. Since 2010 the expertise is also provided to scientists outside the MPIMG and the group acquired the status of a Sequencing Core Facility for all institutes of the biological-medical section of the Max Planck Society. In 2018, the Genomic Unit of the new institute of Emmanuelle Charpentier (Max Planck Unit for the Science of Pathogens) became an integrated part of our facility.
The Sequencing Core Facility operates several next generation sequencers and maintains a fully equipped lab and staff able to perform a variety of sequencing applications - from sample preparation to data analysis. The NGS unit was founded to help researchers process DNA and RNA samples in an efficient and economical manner. We established automation solutions for the sample preparation to increase the throughput and minimize technical variation. By centralizing equipment and expertise, we have dramatically reduced the overall expense to the society, while increasing the efficiency and quality of the data generated.
Currently we are providing expertise for two different technologies: The single molecule, real-time (SMRT) sequencing technology from Pacific Biosciences and the sequencing-by-synthesis technology from Illumina. At a read length of up to 200.000 bases the PacBio technology offers a great benefit especially for de novo genome sequencing, analysis of structural variations, metagenome analysis and full length transcriptome analysis. The high throughput of our Illumina systems completes our sequencing service and offers a real advantage for many applications. Single cell analysis, expression profiling (RNA-Seq), methylation analysis (WGBS, RRBS), copy number analysis as well as the identification of protein binding sides (ChIP-Seq) and the analysis of whole genomes profite from the high output of this technology to a great extent.