Instrumentation

Sequencing Systems

Illumina

NovaSeq 6000

NovaSeq 6000

  • Production-scale system, up to 6000 gigabases and 20 billion reads per run in 2 days

  • maximum read length 2 x 150 bases (paired end)

  • based on a sequencing by synthesis principle and patterned flow cells

  • highly flexible system by multiple flow cell types (S1-S4)

HiSeq 4000

HiSeq 4000

  • high-throughput system, up to 1800 gigabases per run in 3,5 days

  • maximum read length 2 x 150 bases (paired end)

  • based on a sequencing by synthesis principle and patterned flow cells

  • many applications, e.g. genome sequencing, transcriptome sequencing (RNA-Seq) or whole genome bisulfite sequencing

HiSeq 2500 <span lang="en-US">(v4 chemistry adapted)</span>

HiSeq 2500 (v4 chemistry adapted)

  • most flexible high-throughput system, up to 1000 gigabases per run in 7 days

  • maximum read length 2 x 125 bases (paired end, high output mode)

  • 2 x 250 bases read length (rapid mode)

  • based on a sequencing by synthesis principle and random clustered flow cells

  • paired end insert size up to 1.1 kb

NextSeq 500

NextSeq 500

  • very fast system for midsize-range projects, up to 120 gigabases per run in 30 hours

  • maximum read length 2 x 150 bases

  • sequencing by synthesis principle and a dual color chemistry

MiSeq

MiSeq


Pacific Biosciences (PacBio)

PacBio Sequel

PacBio Sequel

  • single molecule system with a read length up to 200 kb

  • sequencing up to 16 SMRT cells per run

  • many applications, e.g. de novo genome sequencing, full length transcriptome analysis or analysis of structural variations


Additional Equipment (Sequencing Pipeline)

BluePippin from <em>Sage Science</em>

BluePippin from Sage Science

Targeted Size Selection for Next-Gen Sequencing (90bp - 50kb)
Spri-Te from <em>Beckman Coulter</em>

Spri-Te from Beckman Coulter

Nucleic Acid Extractor
Biomek NX from <em>Beckmann Coulter</em>

Biomek NX from Beckmann Coulter

384 Channel Liquid Handling System
Nimbus from <em>Hamilton</em>

Nimbus from Hamilton

System for automated emPCR Emulsion Breaking, Enrichment & Primer Annealing for the Roche GS Junior System
LightCycler 480 Real-Time PCR System from <em>Roche</em>

LightCycler 480 Real-Time PCR System from Roche

System for NGS library Quantification
cBot System from <em>Illumina</em>

cBot System from Illumina

Preparation of FlowCells for the HiSeq 4000 System

Equipment for MPIMG Users (supervised by SeqCore)

Chromium Controller from 10x Genomics

Chromium Controller from 10x Genomics

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Location: Tower 4 - Room 0,106
Fragment Analyzer from Advanced Analytical (Agilent)

Fragment Analyzer from Advanced Analytical (Agilent)

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Location: Tower 4 - Room 0,104
2100 Bioanalyzer from <em><span class="copyRight divClear">Agilent Technologies</span></em>

2100 Bioanalyzer from Agilent Technologies

Lab-on-a-Chip nucleic acid and protein analysis with preformatted chips

Location: Tower 4 - Room 0,104
7900HT Real-Time PCR Systems from <em>Applied Biosystems</em>
System for 96-/384-well qPCR

Location: Tower 4 - Room 0,104
<span>QuantStudio 7 Flex Real-Time PCR System from </span><em>Applied Biosystems</em>
Systems for 96/384-well qPCR

Location: Tower 4 - Room 1,103 more
StepOnePlus Real-Time PCR System from <em>Applied Biosystems</em>
System for 96-well qPCR

Location: Tower 4 - Room 1,103
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