Instrumentation

Sequencing Systems

Illumina

NovaSeq 6000

  • Production-scale system, up to 6000 gigabases and 20 billion reads per run in 2 days
  • maximum read length 2 x 150 bases (paired end)
  • based on a sequencing by synthesis principle and patterned flow cells
  • highly flexible system by multiple flow cell types (S1-S4)

NextSeq 500

  • very fast system for midsize-range projects, up to 120 gigabases per run in 30 hours
  • maximum read length 2 x 150 bases
  • sequencing by synthesis principle and a dual color chemistry

MiSeq

iSeq 100

  • smallest Illumina sequencing system
  • yields up to 4mio fragments (8mio PE reads)
  • for library testing or amplicon sequencing

Pacific Biosciences (PacBio)

PacBio Sequel

  • single molecule system with a read length up to 200 kb
  • sequencing up to 16 SMRT cells per run
  • many applications, e.g. de novo genome sequencing, full length transcriptome analysis or analysis of structural variations

Additional Equipment (Sequencing Pipeline)

BluePippin from Sage Science

Targeted Size Selection for Next-Gen Sequencing (90bp - 50kb)

Spri-Te from Beckman Coulter

Nucleic Acid Extractor

Biomek NX from Beckmann Coulter

384 Channel Liquid Handling System

Nimbus from Hamilton

System for automated emPCR Emulsion Breaking, Enrichment & Primer Annealing for the Roche GS Junior System

LightCycler 480 Real-Time PCR System from Roche

System for NGS library Quantification

cBot System from Illumina

Preparation of FlowCells for the HiSeq 4000 System

Equipment for MPIMG Users (supervised by SeqCore)

Chromium Controller from 10x Genomics

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Location: Tower 4 - Room 0,106

Fragment Analyzer from Advanced Analytical (Agilent)

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Location: Tower 4 - Room 0,104

2100 Bioanalyzer from Agilent Technologies

Lab-on-a-Chip nucleic acid and protein analysis with preformatted chips

Location: Tower 4 - Room 0,104
System for 96-/384-well qPCR

Location: Tower 4 - Room 0,104
Systems for 96/384-well qPCR

Location: Tower 4 - Room 1,103 more
System for 96-well qPCR

Location: Tower 4 - Room 1,103
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