Instrumentation

Sequencing Systems

Illumina

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NovaSeq 6000

  • Production-scale system, up to 6000 gigabases and 10 billion fragments per run in 2 days
  • maximum read length 2 x 150 bases (paired end)
  • based on a sequencing by synthesis principle and patterned flow cells
  • highly flexible system by multiple flow cell types (S1-S4)
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NextSeq 2000

  • high-output system, up to 330 GB sequencing output per run in 2 days
  • Maximum read length 2 x 150 b (paired end reads)
  • Integrated data analysis (Illumina DRAGEN Bio-IT Platform)
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NextSeq 500

  • very fast system for midsize-range projects, up to 120 gigabases and 400 million fragments per run in 30 hours
  • maximum read length 2 x 150 bases
  • sequencing by synthesis principle and a dual color chemistry
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MiSeq

  • Flexible benchtop system, up to 15 GB sequencing output
  • up to 25 million fragments per run in 2.5 days
  • Maximum read length 2 x 300 b (paired end reads)

 
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iSeq 100

  • smallest Illumina sequencing system
  • yields up to 4mio fragments (8mio PE reads)
  • for library testing or amplicon sequencing

Pacific Biosciences (PacBio)

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PacBio Sequel II

  • single molecule system with a read length up to 200 kb
  • sequencing up to 16 SMRT cells per run
  • many applications, e.g. de novo genome sequencing, full length transcriptome analysis or analysis of structural variations

Additional Equipment (Sequencing Pipeline)

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BluePippin from Sage Science

Targeted Size Selection for Next-Gen Sequencing (90bp - 50kb)
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Spri-Te from Beckman Coulter

Nucleic Acid Extractor
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Biomek NX from Beckmann Coulter

384 Channel Liquid Handling System
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Nimbus from Hamilton

System for automated emPCR Emulsion Breaking, Enrichment & Primer Annealing for the Roche GS Junior System
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LightCycler 480 Real-Time PCR System from Roche

System for NGS library Quantification

Equipment for MPIMG Users (supervised by SeqCore)

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Chromium Controller from 10x Genomics

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Location: Tower 4 - Room 0,106
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Fragment Analyzer from Advanced Analytical (Agilent)

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Location: Tower 4 - Room 0,104
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2100 Bioanalyzer from Agilent Technologies

Lab-on-a-Chip nucleic acid and protein analysis with preformatted chips

Location: Tower 4 - Room 0,104
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System for 96-/384-well qPCR

Location: Tower 4 - Room 0,104
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Systems for 96/384-well qPCR

Location: Tower 4 - Room 1,103 more
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System for 96-well qPCR

Location: Tower 4 - Room 1,103
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