Our lab proposes two potential projects. One to study how different levels in gene regulatory factors affect cellular decision. The second to study how gene regulatory DNA affects cell-to-cell heterogeneity in transcriptional responses. Both projects will be relevant to better understand gene regulation and cell fate; using CRISPR tools, genomic profiling techniques, and relevant computational analysis.
We scanned the regulatory landscape of Xist and found several proximal and distal enhancers, as well as a new ncRNA Xert, that all control X-inactivation.
Distal and proximal cis-regulatory elements sense X-chromosomal dosage and developmental state at the Xist locus. Gjaltema RAF*, Schwämmle T*, Kautz P, Robson M, Schöpflin R, Lustig LR, Brandenburg L, Dunkel I, Vechiatto C, Ntini E, Mutzel V, Schmiedel V, Marsico A, Mundlos S, Schulz EG.Molecular Cell 2021
...ups, we lost some records here :( please have patience - we are working on recovering them...
Publication - 07.06.2019
Our work together with the Marsico lab, where we use machine learning to identify the rules of Xist-mediated gene silencing was published:
Kinetics of Xist-induced gene silencing can be predicted from combinations of epigenetic and genomic features. Barros de Andrade E Sousa L, Jonkers I, Syx L, Dunkel I, Chaumeil J, Picard C, Foret B, Chen CJ, Lis JT, Heard E, Schulz EG, Marsico A. Genome Res. 2019
Publication - 08.04.2019
First mathematical model of Xist regulatory network published. See press release