Equipment
The Flow Cytometry Lab operates two cell sorters (BD FACSAriaII and BD FACSAria Fusion, FACS room: K4 2.12, phone -1543) and two cell analyser (BD FACSCelesta, Accuri), which are state-of-the-art instruments.
For further details, please see the sorter’s configurations. Note, the Fusion has a UV (355 nm) and a violet (405 nm) laser. So for detection of Dapi, Hoechst or Brilliant UV (BUV)- and BrilliantViolet(BV) dyes, Pacific blue, Pacific Orange, V500, V450..., you can only use the Fusion. Propidium iodide (PI), CFP, YFP, Venus, GFP, mCherry, dTomato, RFP, dsRed, PE, FITC, PerCP, APC, Alexa647, Alexa633, Alexa488, APC-Cy7, Pe-Cy7 among others can be detected on both FACS machines.
![Standard configuration Fusion (FACS AriaFusion 2B-5YG-3R-2UV-6V)](/4039104/original-1711129166.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQwMzkxMDR9--5d1f934e3fc8e5761f40c356450cf919b3af9464)
![CFP configuration Fusion (FACS AriaFusion 2B-5YG-2R-1DB-2UV-6V)](/4039074/original-1711129166.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQwMzkwNzR9--0d89b4217335f46b63c4674dc628446dde26e620)
![Standard configuration Aria II (Aria II SORP 85um, Aria 4-Laser, 12-color (5b-1db-2r-3yg))](/4039147/original-1711129166.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQwMzkxNDd9--2914e6705cefc4f173d6adc41562975dbdc3cc9e)
![Basic configuration Accuri C6 (Accuri C6)](/4039180/original-1711129166.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQwMzkxODB9--fb3e4f2d7bfc620fed5b6b6d6084422b2be0092d)
![original](/4683731/original-1711129165.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQ2ODM3MzF9--4847d259227172766369a68b241a7665b01a9b9f)