Evolution & Development Group
Evolution of regulatory elements
Using a computational method developed in our lab, we identified phylogenetically conserved noncoding elements (PCNEs) in a manner that is not biased by rearrangement and duplication. We identified more than a thousand PCNEs that have been conserved between vertebrates and the basal chordate amphioxus. Via transgenic zebrafish assays we found that the majority of the computationally identified elements are functional enhancers. We could show that PCNEs are enriched around genes with ancient synteny conservation and that this association is strongest for extragenic PCNEs, suggesting that cis-regulatory interdigitation plays a key role in repressing genome rearrangement. Our results also demonstrate that subfunctionalization of conserved cis-regulation has not been the primary determinate of gene duplicate retention in vertebrates. Instead, the data supports the gene balance hypothesis, which proposes that duplicate retention has been driven by selection against dosage imbalances in genes with many protein connections.
![(A) PCNEs associated with the Sox14/21 gene family. Each genomic region is 204 kb long. Genes are shown as arrows indicating the direction of transcription. Black boxes indicate exons. The small single-exon Sox14/21 genes are labeled with red tri- angles. PCNEs are shown as colored lines; the colors and the numbers above the PCNEs indicate the group membership of each PCNE and reveal conservation of PCNE order. Neighboring genes that may represent conserved syntenic relationships are labeled; unlabeled genes are not syntenically conserved. A maximum likelihood phylogenetic tree of the Sox14/21 proteins is shown on the right.(B) A multiple alignment of a portion of the Sox14/21 group 1 PCNEs, with conserved binding motifs highlighted in pink. Bf, amphi- oxus; Tr, fugu; Dr, zebrafish; Mm, mouse](/3825461/original-1517424297.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjM4MjU0NjF9--12d23f5389b35ec34b281e9b4964048a5848d32c)
(A) PCNEs associated with the Sox14/21 gene family. Each genomic region is 204 kb long. Genes are shown as arrows indicating the direction of transcription. Black boxes indicate exons. The small single-exon Sox14/21 genes are labeled with red tri- angles. PCNEs are shown as colored lines; the colors and the numbers above the PCNEs indicate the group membership of each PCNE and reveal conservation of PCNE order. Neighboring genes that may represent conserved syntenic relationships are labeled; unlabeled genes are not syntenically conserved. A maximum likelihood phylogenetic tree of the Sox14/21 proteins is shown on the right.(B) A multiple alignment of a portion of the Sox14/21 group 1 PCNEs, with conserved binding motifs highlighted in pink. Bf, amphi- oxus; Tr, fugu; Dr, zebrafish; Mm, mouse
![Lateral view of zebrafish 24hrs embryos showing the tissue expression of the zebrafish orthologs of the following three human 16p11.2 genes A: DOC2A, B: ypel3, C: ppp4ca genes at 24hrs. Abbreviations: Ce:Cerebellum, Op: oprtical plakode, Mv: ventral Mesencephalon (Tegmentum), Rh: Rhombencephalon, Lp: Linsenplakode](/3825472/original-1517424298.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjM4MjU0NzJ9--d82d9c75e09a6f521aceb27b1f4e9aadcdb971e9)