Transcriptional regulation of adipose stem cell differentiation

Prof. Dr. Sigmar Stricker

October 26, 2020

Our group aims to understand mechanisms controlling differentiation of fibrogenic, adipogenic, and myogenic progenitors in development, as well as the respective adult tissue-resident stem cells (see e.g. these articles in Nature Communications, Development and Stem Cell Research).

This year, we offer 2 PhD projects targeted at unraveling the transcriptional and epigenetic mechanisms governing the differentiation of adipose tissue-resident stem cells as well as muscle stem cells. Both projects will make use of state-of-the-art genome-wide technologies (RNA-Seq, ATAC-Seq, ChIP-Seq), CRISPR-Cas9-mediated gene editing, metabolomics, and single cell sequencing.

Tissue section of developing adipose tissue, Nuclei blue (DAPI), lipids red (OilredO), Osr1 lineage green (Or1Cre;R26mTmG)

This project will utilize embryonic and adult adipose progenitors / stem cells as well as adipogenic cell lines, and ES cell-derived organoid models. Our previous studies suggest a key role for the transcription factor Osr1 in adipose tissue differentiation, and in preservation of adult adipose stem cells by maintaining cells in a poised, but undifferentiated state. We will use a combination of in vitro and in vivo models to manipulate Osr1 expression and analyze its interaction with chromatin modifiers, with the aim to decipher the mechanistic interplay of transcription factor binding and chromatin modification fine-tuning cell differentiation.

Project partner: Sarah Kinkley (MPIMG)

For more information, visit the website of the Biochemistry and Genetics group

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