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Semi-Automated Technologies

Antibody Phage Display

Antibody phage display uses genetically engineered phage, viruses that infect bacteria, to display and produce antibody fragments. This technique was first published by McCafferty, Griffiths, Winter and Chiswell in 1990. Simple peptide phage display was described already two years earlier by Parmley and Smith. The display of immune-libraries on filamentous phage coat proteins has ever since become the method of choice to produce antibodies without hybridoma technology or immunization of animals.

Human antibody phage display libraries contain antibody genes amplified from the B-cells of donor individuals and can be viewed as a mimic of the humoral arm of a human immune system in a test tube.

Scheme taken from TARGETS Vol. 1, No. 1 July 2002

Protein-Microarrays for investigation of human diseases

Protein microarrays can be used to identify and to monitor disease specific antibodies. We have used this technique in the past for autoimmune diseases (RA, SLE, CD, DCM).

For this approach we perform serum-screening experiments using protein macroarrays (22 cm x 22 cm) containing 37,200 redundant, recombinant human proteins. Pre selected proteins will be expressed, purified and used to generate protein microarrays, to perform qualitative and quantitative validation of these putative autoantigens.

last updated: 31 December, 2009