PCR

For the PCR amplification of cDNA clones we are using a robotic system from Tecan (Genesis workstation 200) combined with a temperature-controlled hotel for storage of microtiter plates from Heraeus (Cytomat 6002). External cyclers from MWG biotech (Primus multiblock) are being utilized.

Arrayer

Our lab is equipped with two arrayers for the production of DNA microarrays. One from Beecher Instruments and one from ESI (SDDC-2). Both deposit DNA probes onto glass slides via contact printing with split pins.

Scanner

Three confocal laser scanners are available for the detection of fluorescent signals. An older model from Beecher Instruments, the GMS 418 (Genetic Microsystems) and the Affymetrix 428 Scanner.

cDNA arrays

Human X chromosome-specific cDNA array currently consisting of 4552 IMAGE clones Human blood cDNA array representing 17.013 UniGene clusters with expression in hematopoetic, predominantly lymphatic cells Murine brain-specific cDNA array consisting of 3664 clones Murine eye-specific cDNA array with 5280 clones Murine cDNA array consisting of 20.000 non-redundant clones