Gene expression profile of mouse bone marrow stromal cells determined by cDNA micoarray analysis

¹ Max-Planck-Institute for Molecular Genetics, Ihnestr 73, 14195 Berlin, Germany
² Freie Universität Berlin, Fachbereich Biologie, Chemie, Pharmazie, Takustr 3, 14195 Berlin, Germany
³ Freie Universität Berlin, Research Facility for Experimental Medicine, Krahmerstr 6, 12207 Berlin, Germany

⁴ corresponding author
Ihnestr 73, 14195 Berlin, Germany. Tel. +49 30 8413 1243, Fax +49 30 7413 1383, E-mail: nuber@molgen.mpg.de

ABSTRACT

Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells which are isolated from bone marrow by their selective adherence to plastic remains unkown so far. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared to two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes which encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines / cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.

• Publication
• expressed clones
• highly expressed clones

Christine Steinhoff - Last Change: 03.02.03