Max Planck Institute for Molecular Genetics

Max Planck Institute for Molecular Genetics - Ihnestraße 73 - 14195 Berlin - Germany - Phone: (+49 30) 8413 0 - Fax: (+49 30) 8413 1388
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Molecular Cytogenetics and chromosome evolution

Molecular Cytogenetics

Research Areas

Team

Publications

Haaf T., Schmid M. (1991) Chromosome topology in mammalian interphase nuclei. Exp. Cell Res. 192, 325-332.

Haaf T., Warburton P.E., Willard H.F. (1992) Integration of human alpha-satellite DNA into simian chromosomes: centromere protein binding and disruption of normal chromosome segregation. Cell 70, 681-696.

Haaf T. (1995) The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: implications for methylation-associated cellular processes. Pharmac. Ther. 65, 19-46.

Haaf T., Ward D.C. (1995) Higher-order nuclear structure in mammalian sperm revealed by in situ hybridization and extended chromatin fibers. Exp. Cell Res. 219, 604-611.

Haaf T., Golub E.I., Reddy G., Radding C.M., Ward D.C. (1995) Nuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes. Proc. Natl. Acad. Sci. USA 92, 2298-2302.

Haaf T., Matera A.G., Wienberg J., Ward D.C. (1995) Presence and abundance of CENP-B box sequences in great ape subsets of primate-specific alpha-satellite DNA. J. Mol. Evol. 41, 487-491.

Haaf T. (1996) High-resolution analysis of DNA replication in released chromatin fibers containing 5-bromodeoxyuridine. BioTechniques 21, 1050-1054.

Haaf T., Bray-Ward P. (1996) Region-specific YAC banding and painting probes for comparative genome mapping: implications for the evolution of human chromosome 2. Chromosoma 104, 537-544.

Haaf T., Ward D.C. (1996) Inhibition of RNA polymerase II transcription causes chromatin decondensation, loss of nucleolar structure, and dispersion of chromosomal domains. Exp. Cell Res. 224, 163-173.

Haaf T., Sirugo G., Kidd K.K., Ward D.C. (1996) Chromosomal localization of long trinucleotide repeats in the human genome by fluorescence in situ hybridization. Nature Genet. 12, 183-185.

Kovalenko O.V., Plug A.W., Haaf T., Gonda D.K., Ashley T., Ward D.C., Radding C.M., Golub E.I. (1996) Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes. Proc. Natl. Acad. Sci. USA 93, 2958-2963.

Warburton P.E., Haaf T., Gosden J., Lawson D., Willard H.F. (1996) Characterization of a chromosome-specific chimpanzee alpha satellite subset: evolutionary relationship to subsets on human chromosomes. Genomics 33, 220-228.

Haaf T., Willard H.F. (1997) Chromosome-specific alpha-satellite from the centromere of chimpanzee chromosome 4. Chromosoma 106, 226-232.

Kingsley K., Wirth J., van der Maarel S., Freier S., Ropers H.-H., Haaf T. (1997) Complex FISH probes for the subtelomeric regions of all human chromosomes: comparative hybridization of CEPH YACs to chromosomes of the Old World monkey Presbytis cristata and great apes. Cytogenet. Cell Genet. 78, 12-19.

Koehler M.R., Haaf T., Guttenbach M., Schartl M., Schmid M. (1997) Cytogenetics of the genus Leporinus (Pisces, Anostomidae). II. Molecular cytogenetics, organization and evolutionary conservation of a chromosome-specific satellite DNA from Leporinus obtusidens. Chromosome Res. 5, 325-331.

Golub E.I., Gupta R.C., Haaf T., Wold M.S., Radding C.M. (1998) Interaction of human Rad51 recombination protein with single-stranded DNA binding protein, RPA. Nucleic Acids Res. 26, 5388-5393.

Haaf T., Willard H.F. (1998) Orangutan alpha-satellite monomers are closely related to the human consensus sequence. Mamm. Genome 9, 440-447.

Smith A., Haaf T. (1998) DNA nicks and increased sensitivity of DNA to fluorescence in situ end labeling during functional spermatogenesis. BioTechniques 25, 496-502.

Grützner F., Himmelbauer H., Paulsen M., Ropers H.-H., Haaf T. (1999a) Comparative mapping of mouse and rat chromosomes by fluorescence in situ hybridization. Genomics 55, 306-313.

Grützner F., Lütjens G., Rovira C., Barnes D.W., Ropers H.-H., Haaf T. (1999b) Classical and molecular cytogenetics of the pufferfish Tetraodon nigroviridis. Chromosome Res. 7, 655-662.

Haaf T., Raderschall E., Reddy G., Ward D.C., Radding C.M., Golub E.I. (1999) Sequestration of mammalian Rad51-recombination protein into micronuclei. J. Cell Biol. 144, 11-20.

Hardt T., Himmelbauer H., Mann W., Ropers H.-H., Haaf T. (1999) Towards identification of individual homologous chromosomes: comparative genomic hybridization and spectral karyotyping discriminate between paternal and maternal euchromatin in Mus musculus x M. spretus interspecific hybrids. Cytogenet. Cell Genet. 86, 187-193.

Nanda I., Shan Z., Schartl M., Burt D.W., Koehler M., Nothwang H.G., Grützner F., Paton I.R., Windsor D., Dunn I., Engel W., Staeheli P., Mizuno S., Haaf T., Schmid M. (1999) 300 million years of conserved synteny between chicken Z and human chromosome 9. Nature Genet. 21, 258-259.

Prietz S., Hardt T., Mann W., Haaf T. (1999) FISH und CHIPS. BIOspektrum 3/99, 222-227.

Raderschall E., Golub E.I., Haaf T. (1999) Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage. Proc. Natl. Acad. Sci. USA 96, 1921-1926.

Riesselmann L., Haaf T. (1999) Preferential S-phase pairing of the imprinted region on distal mouse chromosome 7. Cytogenet. Cell Genet. 86, 39-42.

Roemer I., Grützner F., Winking H., Haaf T., Orth A., Skidmore L., Antczak D., Fundele R. (1999) Global methylation in eutherian hybrids. Nature 401, 131-132.

Sharon D., Glusman G., Pilpel Y., Khen M., Gruetzner F., Haaf T., Lancet D. (1999) Primate evolution of an olfactory receptor cluster: diversification by gene conversion and recent emergence of pseudogenes. Genomics 61, 24-36.

Stout K., van der Maarel S., Frants R.R., Padberg G.W., Ropers H.-H., Haaf T. (1999) Somatic pairing between subtelomeric chromosome regions: implications for human genetic disease? Chromosome Res. 7, 323-329.

Wirth J., Nothwang H.-G., van der Maarel S., Menzel C., Borck G., Lopez-Pajares I., Brøndum-Nielsen K., Tommerup N., Bugge M., Ropers H.-H., Haaf T. (1999) Systematic characterization of disease associated balanced chromosome rearrangements by FISH: cytogenetically and genetically anchored YACs identify microdeletions and candidate regions for mental retardation genes. J. Med. Genet. 36, 271-278.

Brunner B., Grützner F., Yaspo M.-L., Ropers H.-H., Haaf T., Kalscheuer V.M. (2000) Molecular cloning and characterization of the Fugu rubripes MEST/COPG2 imprinting cluster and chromosomal localization in Fugu and Tetraodon nigroviridis. Chromosome Res. 8, 465-476.

Cai L., Lindpaintner K., Browne J., Gruetzner F., Haaf T., James M.R., Bihoreau M.-T. (2000) An anchored YAC-STS framework for the rat genome. Cytogenet. Cell Genet. 89, 168-170.

Haaf T. (2000) Fluorescence in situ hybridization, in Encyclopedia of Analytical Chemistry. Volume 1. Nucleic Acids Structure and Mapping, Meyers R.A., Ed., John Wiley & Sons, Chichester, pp. 4984-5006.

Haaf T., Schmid M. (2000) Experimental condensation inhibition in constitutive and facultative heterochromatin of mammalian chromosomes. Cytogenet. Cell Genet. 91, 113-123.

Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schön O., Desario A., Reichelt J., Kauer G., Blöcker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Haaf T., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. (2000) The DNA sequence of human chromosome 21. Nature 405, 311-319.

Mayer W., Smith A., Fundele R., Haaf T. (2000a) Spatial separation of parental genomes in preimplantation mouse embryos. J. Cell Biol. 148, 629-634.

Mayer W., Niveleau A., Walter J., Fundele R., Haaf T. (2000b) Demethylation of the zygotic paternal genome. Nature 403, 501-502.

Mayer W., Fundele R., Haaf T. (2000c) Spatial separation of parental genomes during mouse interspecific (Mus musculus x M. spretus) spermiogenesis. Chromosome Res. 8, 555-558.

Nanda I., Zend-Ajusch E., Shan Z., Grützner F., Schartl M., Burt D.W., Koehler M., Fowler V.M., Goodwin G., Schneider W.J., Mizuno S., Dechant G., Haaf T., Schmid M. (2000) Conserved synteny between the chicken Z sex chromosome and human chromosome 9 includes the male regulatory gene, DMRT1: a comparative (re)view on avian sex determination. Cytogenet. Cell Genet. 89, 67-78.

Nothwang H.-G., Schröer A., van der Maarel S., Kübart S., Schneider S., Rießelmann L., Menzel C., Hinzmann B., Vogt D., Rosenthal A., Fryns J.-P., Tommerup N., Haaf T., Ropers H.H., Wirth J. (2000) Molecular cloning of Xp11 breakpoints in two unrelated mentally retarded females with X;autosome translocations. Cytogenet. Cell Genet. 90, 126-133.

Shan Z., Nanda I., Wang Y., Schmid M., Vortkamp A., Haaf T. (2000a) Sex-specific expression of an evolutionarily conserved male regulatory gene, DMRT1, in birds. Cytogenet. Cell Genet. 89, 252-257.

Shan Z., Zabel B., Trautmann U., Hillig U., Ottolenghi C., Wang Y.Y., Haaf T. (2000b) FISH mapping of the sex-reversal region on human chromosome 9p in two XY females and in primates. Eur. J. Hum. Genet. 8, 167-173.

Borck G., Wirth J., Hardt T., Toennies H., Brøndum-Nielsen K., Bugge M., Tommerup N., Nothwang H.-G., Ropers H.-H., Haaf T. (2001) Molecular cytogenetic characterization of a complex 46,XY,t(7;8;11;13) chromosome rearrangement in a patient with Moebius syndrome. J. Med. Genet. 38, 117-120.

Haaf T. (2001) The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. Chromosome Res. 9, 263-271.

Lapidot M., Pilpel Y., Gilad Y., Falcovitz A., Sharon D., Haaf T., Lancet D. (2001) Mouse-human orthology relationships in an olfactory receptor gene cluster. Genomics 71, 296-306.