Anwenderzentrum zur Analyse von Proteinkomplexen durch Kryo-Elektronenmikroskopie:

2009

Bhushan, S., Gartmann, M., Halic, M., Armache, J.-P., Jarasch, A., Mielke, T., Berninghausen, O., Wilson, D. N. and Beckmann, R. (2009) a-helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel, submitted

Müller, H., Mirgorodskaya, E., Steinbrink, S., Schmidt, D., Lehmann, V., Habermann, K., Gustavsson, N., Kessler, T., Ploubidou, A., Lehrach, H., Gobom, J., Boutros, M., Lange, B.M.H. (2009) Proteomic and functional analysis of the mitotic Drosophila centrosome, in revision

Liu, C., Young, A.L., Starling-Windhof, A., Bracher, A., Saschenbrecker, S., Vasudeva Rao, B., Vasudeva Rao, K., Berninghausen, O., Mielke, T., Hartl, F.U., Beckmann, R. & Hayer-Hartl, M. (2009) Coupled chaperone action in folding and assembly of hexadecameric Rubisco. Nature, under revision

Becker, T., Mandon, E., Bhushan, S., Jarasch, A., Armache, J.-P., Funes, S., Jossinet, F., Gumbart, J. C., Mielke, T., Schulten, K., Westhof, E., Gilmore, R. and Beckmann, R. (2009) Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Science,29 October 2009 (DOI: 10.1126/science.1178535)

Seidelt, B., Innis, C.A., Wilson, D.N., Gartmann, M., Armache, J-P., Trabuco, L.G., Becker, T., Mielke, T., Schulten, K., Steitz, T.A. and Beckmann, R. (2009) Structural insight into nascent chain-mediated translational stalling. Science, 29 October 2009 (DOI: 10.1126/science.1177662)

Spahn, C.M.T. & Penczek, P.A. (2009) Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM. Current Opinion Struct. Biol. 19, 623-631

Schuette, J.-C., Murphy, F.V. 4th, Kelley, A.C., Giesebrecht, J., Connell, S.R., Loerke, J., Mielke, T., Zhang, W., Penczek, P.A., Ramakrishnan, V. and Spahn, C.M.T. (2009) GTPase activation of elongation factor EF-Tu by the ribosome during decoding. EMBO J. 28, 755-765

2008

Zhang, W., Kimmel., M., Spahn, C.M.T., Penczek, P.A. (2008) Heterogeneity of large macromolecular complexes revealed by 3D cryo-EM variance analysis. Structure 16, 1770-6

Connell, S.R., Topf, M., Qin, Y., Wilson, D.N., Mielke, T., Fucini, P., Nierhaus, K.H. and Spahn, C.M.T. (2008) A novel tRNA-intermediate revealed on the ribosome during EF4 (LepA)-mediated back-translocation. Nature Structural & Molecular Biology 15, 910-915

Harms, J.M., Wilson, D.N., Schluenzen, F., Connell, S.R., Stachelhaus, T., Zaborowska, Z., Spahn, C.M.T., Fucini, P. (2008) Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin. Mol Cell. 30, 26-38

Chen, J., Sachse, C., Xu, C., Mielke, T., Spahn, C.M.T. and Grigorieff, N. (2008) A Dose-Rate Effect in Single-Particle Electron Microscopy. J. Struc. Biol. 161, 92-100

2007

Kuhn, C.-D., Geiger, S.R., Baumli, S., Gartmann, M., Gerber, J., Jennebach, S., Mielke, T., Tschochner, H., Beckmann, R., and Cramer, P. (2007) Functional architecture of RNA polymerase I. Cell 131, 1260-1272,

Varmark H., Llamazares S., Rebollo E., Lange B.M.H., Reina J., Schwarz H., Gonzalez C. (2007) Asterless Is a Centriolar Protein Required for Centrosome Function and Embryo Development in Drosophila. Current Biology ; 17(20):1735-1745,

Connnell, S.R., Takemoto, C., Wilson, D.N., Wang, H., Murayama, K., Terada, T., Shirouzu, M., Rost, M., Schüler, M., Giesebrecht, J., Dabrowski, M., Mielke, T., Fucini, P.M., Yokoyama, S. and Spahn, C.M.T. (2007) Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors. Molecular Cell 25, 751-764

2006

Schüler, M., Connell, S.R., Lescoute, A., Giesebrecht J., Dabrowski, M., Schroeer, B., Mielke, T., Penczek, P.A., Westhof, E., Spahn, C.M.T. (2006) Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nature Structural & Molecular Biology 13, 1092-1096