The markers I put into the EUCIB database are in fact protein spots on two-dimensional electrophoresis gels. They all start with "JK" for J. Klose, in whose lab the corresponding gels were made.
Prof. Joachim Klose
joachim.klose@charite.de
When comparing 2D protein gels from Mus spretus, Black 6 and their F1 progeny, certain spots appear heterozygous between the parent strains. Gels of 64 EUCIB backcross mice tissues have been used to derive allele data for heterozygous spots; this data was then entered into the database.
The tissue used was always brain.
Different kinds of heterozygosity can be differentiated:
The purpose of this project was to add mapping information to protein spots. While some of the spots are known proteins, most of them are anonymous. It has to be noted that generally variations on the protein level may well be influenced by more then one locus. Indeed there are quite a few spots which do not show convincing linkage to any single anchor marker.
Right now, many proteins corresponding to the entered data are completely uncharacterized and no sequence information is available. Hopefully the planned characterization of the protein spots by mass spectrometry and/or Edmann-degradation will soon be under way. This should allow matching the protein spots with cDNA sequences and thereby generate sequence tags.
Summarizing, I fear that the markers I have entered may not
be of too much use to other people's mapping endevours right
now, the proteins still being uncharacterized.
Still there is
another possibility: The protein-expression
patterns of affected tissues from disease carrying mice
can be analysed by 2D-electrophoresis and compared to wildtype.
The changed protein expression and the genetic mapping
of the proteins may be correlated to genetic mapping information of the disease.