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Evolutionary Genomics Group Department of Computational Molecular Biology |
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Title: A calibrated diversity assay for nucleic acid libraries using DiStRO--a Diversity Standard of Random Oligonucleotides. Authors: Tatjana Schütze, Peter F Arndt, Marcus Menger, Aniela Wochner, Martin Vingron, Volker A Erdmann, Hans Lehrach, Christian Kaps, Jörn Glökler Abstract: We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations. Reference: Nucleic Acids Research 38 (2010) e23 Links: [Journal] [Fulltext (pdf)] |