Selected References

  1. Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH. The Highly Conserved LepA Is a Ribosomal Elongation Factor that Back-Translocates the Ribosome. Cell 127:721-733 (2006)

  2. Schluenzen F, Takemoto C, Wilson DN, Kaminishi T, Harms JM, Hanawa-Suetsugu K, Szaflarski W, Kawazoe M, Shirouzu M, Nierhaus KH, Yokoyama S, Fucini P. The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation. Nat. Struct. Mol. Biol. 13:871-878 (2006)

  3. Iskakova MB, Szaflarski W, Dreyfus M, Remme J, Nierhaus KH. Troubleshooting coupled in vitro transcription-translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins. Nucleic Acids Res. 34:e135 (2006)

  4. Wilson DN, Nierhaus KH. The E-site story: the importance of maintaining two tRNAs on the ribosome during protein synthesis.
    Cell Mol. Life Sci. 63:2725-2737 (2006)

  5. Nierhaus KH. Decoding errors and the involvement of the E-site.
    Biochimie. 88:1013-1019 (2006)

  6. Seo HS, Abedin S, Kamp D, Wilson DN, Nierhaus KH, Cooperman BS. EF-G-dependent GTPase on the ribosome conformational change and fusidic acid inhibition. Biochemistry 45:2504-2514 (2006)

  7. Wilson DN, Harms JM, Nierhaus KH, Schlunzen F, Fucini P. Species-specific antibiotic-ribosome interactions: implications for drug development. Biol Chem. 386:1239-1252 (2005)

  8. Wilson DN, Nierhaus KH. Ribosomal proteins in the spotlight.
    Crit. Rev. Biochem. Mol. Biol. 40:243-267 (2005)

  9. Dinos G, Kalpaxis DL, Wilson DN, Nierhaus KH. Deacylated tRNA is released from the E site upon A site occupation but before GTP is hydrolyzed by EF-Tu.
    Nucleic Acids Res. 33:5291-5296 (2005)

  10. Ilag LL, Videler H, McKay AR, Sobott F, Fucini P, Nierhaus KH, Robinson CV. Heptameric (L12) 6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria. Proc. Natl. Acad. Sci. USA 102:8192-8197 (2005)

  11. Wilson DN, Nierhaus KH. RelBE or not to be.
    Nat. Struct. Mol. Biol. 12:282-284 (2005)

  12. Shpanchenko OV, Zvereva MI, Ivanov PV, Bugaeva EY, Rozov AS, Bogdanov AA, Kalkum M, Isaksson LA, Nierhaus KH, Dontsova OA.Stepping transfer messenger RNA through the ribosome. J. Biol. Chem. 280:18368-18374 (2005)

  13. Christodoulou J, Larsson G, Fucini P, Connell SR, Pertinhez TA, Hanson CL, Redfield C, Nierhaus KH, Robinson CV, Schleucher J, Dobson CM. Heteronuclear NMR investigations of dynamic regions of intact Escherichia coli ribosomes. Proc. Natl. Acad. Sci. USA 101:10949-10954 (2004)

  14. Marquez V, Wilson DN, Tate WP, Triana-Alonso F, Nierhaus KH. Maintaining the ribosomal reading frame: the influence of the E site during translational regulation of release factor 2. Cell 118:45-55 (2004)

  15. Dinos G, Wilson DN, Teraoka Y, Szaflarski W, Fucini P, Kalpaxis D and Nierhaus KH: Dissecting the ribosomal inhibition mechanisms of edeine and pactamycin: The universally conserved residues G693 and C795 regulate P-site RNA binding. Mol. Cell 13:113-124 (2004)

  16. El'skaya AV, Ovcharenko GV, Palchevskii SS, Petrushenko ZM, Triana-Alonso FJ and Nierhaus KH: Three tRNA binding sites in rabbit liver ribosomes and role of the intrinsic ATPase in 80S ribosomes from higher eukaryotes. Biochemistry 36:10492-10497 (1997)

  17. Triana-Alonso FJ, Chakraburtty K and Nierhaus KH: The elongation factor 3 unique in higher fungi and essential for protein biosynthesis is an E site factor. J. Biol. Chem. 270:20473-20478 (1995)

  18. Lewicki BTU, Margus T, Remme J and Nierhaus KH: Coupling of rRNA transcription and ribosomal assembly in vivo: Formation of active ribosomal subunits in Escherichia coli requires transcription of rRNA genes by host RNA polymerase which cannot be replaced by T7 RNA polymerase. J. Mol. Biol. 231:581-593 (1993)

  19. Geigenmüller U and Nierhaus KH: Significance of the third tRNA binding site, the E site, on E. coli ribosomes for the accuracy of translation: an occupied E site prevents the binding of non-cognate aminoacyl-tRNA to the A site. EMBO J. 9:4527-4533 (1990)

  20. Franceschi FJ and Nierhaus KH: Ribosomal proteins L15 and L16 are mere late assembly proteins of the large ribosomal subunit. J. Biol. Chem. 265:16676-16682 (1990)

  21. Hausner Th-P, Geigenmüller U and Nierhaus KH: The allosteric 3-site model for the ribosomal elongation: New insight in the inhibition mechanisms of aminoglycosides, viomycin and thiostrepton. J. Biol. Chem. 263:13103-13111 (1988)

  22. Nowotny V and Nierhaus KH: Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains, which are initiated by S4 and S7. Biochemistry 27:7051-7055 (1988)

  23. Herold M and Nierhaus KH: Incorporation of six additional proteins to complete the assembly map of the 50S subunit from Escherichia coli ribosomes. J. Biol. Chem. 262:8826-8833 (1987)

  24. Rheinberger H-J and Nierhaus KH: Allosteric interactions between the ribosomal transfer RNA-binding sites A and E. J. Biol. Chem. 261:9133-9139 (1986)

  25. Saruyama H and Nierhaus KH: Evidence that the three-site model for the ribosomal elongation cycle is also valid in the archaebacterium Halobacterium halobium. Mol. Gen. Genet. 204:221-228 (1986)

  26. Rheinberger H-J and Nierhaus KH: Testing an alternative model for the ribosomal peptide elongation. Proc. Natl. Acad. Sci. USA 80:4213-4217 (1983)

  27. Nowotny V and Nierhaus KH: Initiator proteins for the assembly of the 50S subunit from Escherichia coli ribosomes. Proc. Natl. Acad. Sci. USA 79:7238-7242 (1982)

  28. Rheinberger H-J, Sternbach H and Nierhaus KH: Three tRNA binding sites on Escherichia coli ribosomes. Proc. Natl. Acad. Sci. USA 78:5310-5314 (1981)

  29. Spillmann S and Nierhaus KH: The ribosomal protein L24 of Escherichia coli is an assembly protein. J. Biol. Chem. 253:7047-7050 (1978)

  30. Sieber G and Nierhaus KH Kinetic and thermodynamic parameters of the assembly in vitro of the large subunit from Escherichia coli ribosomes. Biochemistry 17:3505-3511 (1978)

  31. Spillmann S, Dohme F and Nierhaus KH: Assembly in vitro of the 50S subunit from Escherichia coli ribosomes: Proteins essential for the first heat dependent conformational change. J. Mol. Biol. 115:513-523 (1977)

  32. Dohme F and Nierhaus KH: Total reconstitution and assembly of the 50S subunit from Escherichia coli. J. Mol. Biol. 107:585-599 (1976)

  33. Nierhaus KH and Dohme F.: Total reconstitution of functionally active 50S ribosomal subunits from Escherichia coli. Proc. Natl. Acad. Sci. USA 71:4713-4717 (1974)