Publications:

2005

Wilson DN, Harms JM, Nierhaus KH, Schlunzen F, Fucini P. Species-specific antibiotic-ribosome interactions: implications for drug development. Biol Chem. 2005 Dec;386(12):1239-52. (cover illustration)

Schlunzen F, Wilson DN, Tian P, Harms JM, McInnes SJ, Hansen HA, Albrecht R, Buerger J, Wilbanks SM, Fucini P. The binding mode of the trigger factor on the ribosome: implications for protein folding and SRP interaction. Structure. 2005 Nov;13(11):1685-94.

Wilson DN, Nierhaus KH. Ribosomal proteins in the spotlight. Crit Rev Biochem Mol Biol. 2005 Sep-Oct;40(5):243-67.

Dinos G, Kalpaxis DL, Wilson DN, Nierhaus KH. Deacylated tRNA is released from the E site upon A site occupation but before GTP is hydrolyzed by EF-Tu. Nucleic Acids Res. 2005 Sep 15;33(16):5291-6.

Diaconu M. , Kothe U, Schlunzen F, Fischer N, Harms JM, Tonevitsky AG, Stark H, Rodnina MV, Wahl MC. Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation. Cell. 2005 Jul 1;121(7):991-1004

Ilag L.L, Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H., and Robinson C.V. (2005) Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria. Proc. Natl Acad. Sci. USA 102(23): 8192-8197.

Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, and Agrawal, RK.
Interaction of the highly conserved bacterial GTPase, Era, with the 30S ribosomal subunit: Functional implications for small subunit assembly
Mol. Cell, 2005, April 29; 18(3):
--> Press Release (MPIMG 28.04.2005)

Wilson DN and Nierhaus KH.
RelBE or not to be.
Nature Struct. Mol. Biol. 2005 April; 12(4): 282-4. News and Views

Giavalisco P, Wilson DN, Kreitler T, Lehrach H, Klose J, Gobom J and Fucini P.
High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome
Plant Mol. Biol. 2005, March; 57: 577-591

Mirgorodskaya E, Braeuer C, Fucini P, Lehrach H, Gobom J.
Nanoflow liquid chromatography coupled to matrix-assisted laser desorption/ionization mass spectrometry: sample preparation, data analysis, and application to the analysis of complex peptide mixtures.
Proteomics 2005 Feb; 5(2): 399-408.

Wilson DN, Schluenzen F, Harms JM, Yoshida T, Ohkubo T, Albrecht R, Buerger J, Kobayashi Y, and Fucini P
X-ray crystallography study on ribosome recycling: the mechanism of binding and action of RRF on the 50S ribosomal subunit.
EMBO J. 2005 Jan 26; 24(2): 251-60
--> Press Release (MPIMG 25.04.2005)
--> HASYLAB Newsletter (01.03.2005)

2004

Schlunzen F, Pyetan E, Fucini P, Yonath A, Harms JM.
Inhibition of peptide bond formation by pleuromutilins: the structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with tiamulin.
Mol. Microbiol. 2004 Dec; 54(5): 1287-94. (open access)

Wilson DN and Nierhaus KH.
The how and Y of cold shock.
Nature Struct. Mol. Biol. 2004 Nov; 11(11): 1026-8. News and Views.

Christodoulou J, Larsson G, Fucini P, Connell SR, Pertinhez TA, Hanson CL, Redfield C, Nierhaus KH, Robinson CV, Schleucher J, Dobson CM.
Heteronuclear NMR investigations of dynamic regions of intact Escherichia coli ribosomes.
Proc. Natl Acad. Sci. USA 2004 Jul 27; 101(30): 10949-54.

Márquez V, Wilson DN, Tate WP, Triana-Alonso F, Nierhaus KH.
Maintaining the ribosomal reading frame: The influence of the E site during translational regulation of release factor 2.
Cell, 2004, July 9; 117(8): 1-12.
--> Press Release (MPIMG 12.07.2004)

Petropoulos AD, Xaplanteri MA, Dinos GP, Wilson DN, Kalpaxis DL.
Polyamines affect diversely the antibiotic potency: insight gained from kinetic studies of the blasticidin S and spiramycin interactions with functional ribosomes.
J. Biol. Chem. 2004, Jun 18; 279(25): 26518-25.

Gilbert RJ, Fucini P, Connell S, Fuller S, Nierhaus K, Robinson CV, Dobson CM, Stuart DI.
Three-dimensional structures of translating ribosomes by Cryo-EM.
Mol. Cell 2004 Apr 9; 14(1): 57-66.

Harms JM, Schlunzen F, Fucini P, Bartels H, Yonath A.
Alterations at the peptidyl transferase centre of the ribosome induced by the synergistic action of the streptogramins dalfopristin and quinupristin.
BMC Biol. 2004 Apr 1; 2(1): 4. (open access)
--> Press Release (MPG 20.04.2004)

Dinos G, Wilson DN, Teraoka Y, Szaflarski W, Fucini P, Kalpaxis D, Nierhaus KH.
Dissecting the ribosomal inhibition mechanisms of edeine and pactamycin: the universally conserved residues G693 and C795 regulate P-site RNA binding.
Mol. Cell 2004 Jan 16; 13(1): 113-24.

Sharma MR, Barat C, Wilson DN, Booth TM, Hori-Takemoto C, Yokoyama S, Fucini P, and Agrawal, RK
ERA protein binds in a functionally important region of the 30S ribosomal subunit
Biophys. J. 2004, 86(1): 316A-317A (Part 2 Suppl. S)

2003

Bashan A., Zarivach R., Schluenzen F., Agmon I., Harms J., Auerbach T., Baram D., Berisio R., Bartels H., Hansen H.A., Fucini P., Wilson D., Peretz M., Kessler M., and A. Yonath.
Ribosomal crystallography: peptide bond formation and its inhibition.
Biopolymers 70(1): 19-41 (2003)

Berisio R., Harms J., Schluenzen F., Zarivach R., Hansen H.A., Fucini P., and A. Yonath.
Structural insight into the antibiotic action of telithromycin against resistant mutants.
J. Bacteriol. 185(14): 4276-9 (2003)

Agmon I., Auerbach T., Baram D., Bartels H., Bashan A., Berisio R., Fucini P., Hansen H.A., Harms J., Kessler M., Peretz M., Schluenzen F., Yonath A., and R. Zarivach.
On peptide bond formation, translocation, nascent protein progression and the regulatory properties of ribosomes.
Eur. J. Biochem. 270(12): 2543-2556 (2003)

Hanson C.L., Fucini P., Ilag L.L., Nierhaus K.H., and C.V. Robinson. Dissociation of intact Escherichia coli ribosomes in a mass spectrometer. Evidence for conformational change in a ribosome elongation factor G complex.
J. Biol. Chem. 278(2): 1259-1267 (2003)

Poole ES, Askarian-Amiri ME, Major LL, McCaughan KK, Scarlett DJ, Wilson DN, Tate WP.
Molecular mimicry in the decoding of translational stop signals.
Prog. Nucleic Acid Res. Mol. Biol. 2003;74:83-121. Review.

Wilson DN, Nierhaus KH.
The Ribosome through the Looking Glass.
Angew. Chem. Int. Ed. Engl. 2003 Aug 4;42(30):3464-86.

Blaha G, Wilson DN, Stoller G, Fischer G, Willumeit R, Nierhaus KH.
Localization of the trigger factor binding site on the ribosomal 50S subunit.
J. Mol. Biol. 2003 Feb 21;326(3):887-97.

Scarlett DJ, McCaughan KK, Wilson DN, Tate WP.
Mapping functionally important motifs SPF and GGQ of the decoding release factor RF2 to the Escherichia coli ribosome by hydroxyl radical footprinting. Implications for macromolecular mimicry and structural changes in RF2.
J. Biol. Chem. 2003 Apr 25;278(17):15095-104.